MCCC2 (NM_022132) Human 3' UTR Clone

CAT#: SC216796

3' UTR clone of methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (MCCC2) nuclear gene encoding mitochondrial protein for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MCCC2
• Synonyms: MCCB; MCCCbeta
• ACCN: NM_022132
• Insert Size: 1876 bp
• Sequence Data:
  Insert Sequence

  >SC216796 3’UTR clone of NM_022132
  The sequence shown below is from the reference sequence of NM_022132. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAGACTGACTTCGGTATCTTCAGGATGTAACTGGAATAAAGGATGTTTTCTGTTGGACATGTACTGAAA
  ATTAACACATGTAGTAGCCTTAAAATTTTAGACTTCTCGAACATGAGGCTGTTACAGTAATTTTTTTAA
  CACTGTGCATTGTACTTTTCTACCTTAAAAAAATCAGTGAGGATATTTATTTAATGAACATCAATTCCT
  TTTAAATTTTCTTAGAGAAATTTCTCTGTGGCTCAGTTTTACCACCCATAAAGCGGAGACAGTAATTTA
  CGGTTATCCTTTCTGACCCACAAAGTATGAAAAGTTCTGTAATCTGTAAACTCAGTTCTGTAATCTGTA
  TTATTGAGATGATTAATATAAAGTTGTATTTTCACTGAAATGATTGTTTTGCTGGTTATGCTTGGTGAT
  ATTTTAGCGGGCTTATTTTTGAAAGGCATCTGTTACTTCAGTGGCATAAAGTGCCCTCACACTGCTGTG
  CAGCCATCACCACCATTCATCTCCAGAATTTGTTCTCAGTCCCAAACTGAAACTATACCATTCAAACAA
  CAGCGCTCCCCATTTCCCCCTCCCCCGAGCCCATGACATCTCCTTATTATTATTTTTTTTTTGAGACAG
  GGTGTTTCTCTGTTGCCCAGGCTGGAGTGCGGTGGTGTGATCTCGGCTCACTGCAGCTTCCACCTCCCG
  GGTTCAAGCGATTCTTGTGCCTCAGCCTCCCAAGCAGCTGGGACTATAGGCACGCACCACCACACCCAG
  CTAAGTTCTGTATTTTTAGCAGAGACGGGATTTCACTAGGTTGCCCAGGCTGGTCTCAAACTACTGACC
  TCAAGTGATCCACCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCGCCTGACCA
  CATCTCCTTATTCTTAAATGTATGTGTTTCTCATGGTTTGATTTATTCTTGCTGGGCCATTTCAGCCCC
  TGTCATGGCTTCACTCGCCATCTAAATGCAGATGATTACTGCCTTGACGGTTCTTAACCCAGCTCTCCC
  CTGAGCTGCAGGCCTGCATATCCAGTAGGTCTACTGGACATCTGTACTGGTTGTTGTGGAGGAACCTCT
  GGCTTGCTCATTAAGTCCTACTGATTTTCACTATCCCCTGAATTTCCCCACTTATTTTTGTCTTTCACT
  ATCGCAGGCCTTAGAAGAGGTCTACCTGCCTCCAGTCTTACCTAGTCCAGTCTACCCCCTGGAGTTAGA
  ATGGCCATCCTGAAGTGAAAAGTAATGTCAGATTACTCCCTTCAGTGATTTCTTGTAGAAGTGCCAATC
  CCTGAATGCCACCAAGATCTTAATCTTCACATCTTTAATCTTATCTCTTTGACTCCTCTTTACACCGGA
  GAACGGCTCCAGCTGTTCTAGCTCTCTTTCAGTTCTTTGAACCTTCCCACCTTAGGGTCTATAAGGTTC
  CCTCTGCCCAAATTGTTCTACTCTCCCTTCTTCTTCAACACATCCTTCAGTTTAAGCACTTGCTTCTCT
  CAGTTTAAACTCCACTTCCTTAGGGATGTCTCTGTGACCTCCCTGTGCTGGATTAGCTTCCCTACTCTA
  TGCTCTTGTAAAACACTATCTACTTCCTTTGTCATAAACTCATCATTTTCCTATAAACATTAATATTGT
  CCTCCTGCTAGAATGTAAGCTCCGTGAGAGCAAGGATCCTCCTGTTTACCCTGTACCTCCAATGTCTGG
  CACTTGTAGGTGCTCAAATATTCGTTGAATGAATGAAAAATCCATATTGTAATTGATGTCCTCTGGCCA
  CATAGTTTTAAAATTAGGTGATTGATTATATGACCGAATAGAACTATCAAATGTTTTCCTAATAAAGTC
  AATATTTCAAACA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_022132.5
• Summary: This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]
• Locus ID: 64087
• MW: 69.6