WWP2 (NM_199424) Human 3' UTR Clone

CAT#: SC216628

3' UTR clone of WW domain containing E3 ubiquitin protein ligase 2 (WWP2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: WWP2
• Synonyms: AIP2; WWp2-like
• ACCN: NM_199424
• Insert Size: 1829 bp
• Sequence Data:
  Insert Sequence

  >SC216628 3’UTR clone of NM_199424
  The sequence shown below is from the reference sequence of NM_199424. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GAGGAGACCGAGGGCTTTGGACAGGAGTAACCGAGGCCGCCCCTCCCACGCCCCCCAGCGCACATGTAG
  TCCTGAGTCCTCCCTGCCTGAGAGGCCACTGGCCCCGCAGCCCTTGGGAGGCCCCCGTGGATGTGGCCC
  TGTGTGGGACCACACTGTCATCTCGCTGCTGGCAGAAAAGCCTGATCCCAGGAGGCCCTGCAGTTCCCC
  CGACCCGCGGATGGCAGTCTGGAATAAAGCCCCCTAGTTGCCTTTGGCCCCACCTTTGCAAAGTTCCAG
  AGGGCTGACCCTCTCTGCAAAACTCTCCCCTGTCCTCTAGACCCCACCCTGGGTGTATGTGAGTGTGCA
  AGGGAAGGTGTTGCATCCCCAGGGGCTGCCGCAGAGGCCGGAGACCTCCTGGACTAGTTCGGCGAGGAG
  ACTGGCCACTGGGGGTGGCTGTTCGGGACTGAGAGCGCCAAGGGTCTTTGCCAGCAAAGGAGGTTCTGC
  CTGTAATTGAGCCTCTCTGATGATGGAGATGAAGTGAAGGTCTGAGGGAGCGGGCCCTGGGGCGAGGCC
  ATCTCTGCCTGCCTCCCTAGCAGGCGCCAGCGGTGGAGGCTGAGTCGCAGGACACATGCCGGCCAGTTA
  ATTCATTCTCAGCCAATGAAGGTTTGTCTAAGCTGCCTGGGTATCCACGGGACAAAAACAGCAAACTCC
  CTCAGACTTTGTCCATGTATAAACTTGAAGTGGTTGTGTTGTAGGGTTGCAGGTTTTTTGTTACGCTGC
  TGTCACTTTCTGTCCAGGAGCTGGCACCCCAGGTGTTCTGAGACCTTGAGGGACCCAGACCTTTGGGTC
  CAAGAGTTTCCCAAACAGCCACGCCTCTCAGGAACCCACCTGGCGGTTCCGTGAGCTCAGGCAGGCCTG
  ACCCGGCGGCACAGCCTGGCAGGGACCTCGTCCCCAAGCCTGGCAGAATGAGAGGGGTTGAGGTCCCGA
  GCGCCACTCCTAGCCTTGCCGCCTTCAATAGAGAAGAAATCCCTTTGCTAGATAGGGTCCCCCAGGCAG
  TCCCCAGTGGCGGGACACAGGGGTCCGGCTGTGGAGCTCCCCTGCCAGCCCCTGGAGCTCCAGGAGGGC
  CTGTTGGTCCCCTGTTCAGAATGGAGTGCAGCCCGCCAGCGGAAAGTGTTCATTCTGCATAGGTGTGAG
  GCTTTATCTGCACACAGGACATGAAAACCAGCAGAAAGGCCCTGAGCTGCTGCATAGCCCCATCTGATT
  TCTGCAGTTCCCGCCAGCCTCCAACACGGGGACTCTGCCGTAACTGGAATCTTCATAGGTCATATTGAA
  ATCTTCAAGGTGACCATGCCCCACCGGGGTGCTGGGGCAGTAGTCATGGCAGACTCCCGGCCTGGGCCC
  CCAGGATTCTAGGACCCCCAGGCAGCCCCTTGGACTGGTCCCGGGTGCCTTCCAAGCACAGTCTCCATG
  CTCCCAGATTCTCGACCTTCCCCCGGCCCGGGAGGTGCAGCCTGCGTCTGCCTCTGTCGTGTGTGCTGA
  TTTGAGTGGCTTAGCTTGCCACAGCGCAGCCTCTTCTGTCCCTTTCAGTCATTTGCTGTACTTCCCTGT
  GGCACGTTACCATGGAAGCCGCTCCAGGGTGGGTCAGGGTGCAAGCTGCTGGTGAGGTTTGGAAGCATC
  AGGCTCACGGGTGTTCATGTGTGTTCGTGCGTGTGTGTGCGTACGTGTATATAACTGAAGTGTCTGTAC
  GGAATGCCCTTTGCTAGCCATGGGCTGGTCACCAGATTGTTTTGTAATGCCCGCCCCTTGCCTCGATAT
  TGCCAGTTTCTTGTGCAATAAACAATCAGCAGCTG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_199424.3
• Summary: This gene encodes a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. The encoded protein contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 10. [provided by RefSeq, Jul 2012]
• Locus ID: 11060
• MW: 65.8