Factor VIII (F8) (NM_000132) Human 3' UTR Clone

CAT#: SC216618

3' UTR clone of coagulation factor VIII procoagulant component (F8) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: F8
• Synonyms: AHF; DXS1253E; F8B; F8C; FVIII; HEMA
• ACCN: NM_000132
• Insert Size: 1835 bp
• Sequence Data:
  Insert Sequence

  >SC216618 3’UTR clone of NM_000132
  The sequence shown below is from the reference sequence of NM_000132. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTGGGCTGCGAGGCACAGGACCTCTACTGAGGGTGGCCACTGCAGCACCTGCCACTGCCGTCACCTCTC
  CCTCCTCAGCTCCAGGGCAGTGTCCCTCCCTGGCTTGCCTTCTACCTTTGTGCTAAATCCTAGCAGACA
  CTGCCTTGAAGCCTCCTGAATTAACTATCATCAGTCCTGCATTTCTTTGGTGGGGGGCCAGGAGGGTGC
  ATCCAATTTAACTTAACTCTTACCTATTTTCTGCAGCTGCTCCCAGATTACTCCTTCCTTCCAATATAA
  CTAGGCAAAAAGAAGTGAGGAGAAACCTGCATGAAAGCATTCTTCCCTGAAAAGTTAGGCCTCTCAGAG
  TCACCACTTCCTCTGTTGTAGAAAAACTATGTGATGAAACTTTGAAAAAGATATTTATGATGTTAACAT
  TTCAGGTTAAGCCTCATACGTTTAAAATAAAACTCTCAGTTGTTTATTATCCTGATCAAGCATGGAACA
  AAGCATGTTTCAGGATCAGATCAATACAATCTTGGAGTCAAAAGGCAAATCATTTGGACAATCTGCAAA
  ATGGAGAGAATACAATAACTACTACAGTAAAGTCTGTTTCTGCTTCCTTACACATAGATATAATTATGT
  TATTTAGTCATTATGAGGGGCACATTCTTATCTCCAAAACTAGCATTCTTAAACTGAGAATTATAGATG
  GGGTTCAAGAATCCCTAAGTCCCCTGAAATTATATAAGGCATTCTGTATAAATGCAAATGTGCATTTTT
  CTGACGAGTGTCCATAGATATAAAGCCATTTGGTCTTAATTCTGACCAATAAAAAAATAAGTCAGGAGG
  ATGCAATTGTTGAAAGCTTTGAAATAAAATAACAATGTCTTCTTGAAATTTGTGATGGCCAAGAAAGAA
  AATGATGATGACATTAGGCTTCTAAAGGACATACATTTAATATTTCTGTGGAAATATGAGGAAAATCCA
  TGGTTATCTGAGATAGGAGATACAAACTTTGTAATTCTAATAATGCACTCAGTTTACTCTCTCCCTCTA
  CTAATTTCCTGCTGAAAATAACACAACAAAAATGTAACAGGGGAAATTATATACCGTGACTGAAAACTA
  GAGTCCTACTTACATAGTTGAAATATCAAGGAGGTCAGAAGAAAATTGGACTGGTGAAAACAGAAAAAA
  CACTCCAGTCTGCCATATCACCACACAATAGGATCCCCCTTCTTGCCCTCCACCCCCATAAGATTGTGA
  AGGGTTTACTGCTCCTTCCATCTGCCTGACCCCTTCACTATGACTACACAGAATCTCCTGATAGTAAAG
  GGGGCTGGAGACAAGGATAAGTTATAGAGCAGTTGGAGGAAGCATCCAAAGATTGCAACCCAGGGCAAA
  TGGAAAACAGGAGATCCTAATATGAAAGAAAAATGGATCCCAATCTGAGAAAAGGCAAAAGAATGGCTA
  CTTTTTTCTATGCTGGAGTATTTTCTAATAATCCTGCTTGACCCTTATCTGACCTCTTTGGAAACTATA
  ACATAGCTGTCACAGTATAGTCACAATCCACAAATGATGCAGGTGCAAATGGTTTATAGCCCTGTGAAG
  TTCTTAAAGTTTAGAGGCTAACTTACAGAAATGAATAAGTTGTTTTGTTTTATAGCCCGGTAGAGGAGT
  TAACCCCAAAGGTGATATGGTTTTATTTCCTGTTATGTTTAACTTGATAATCTTATTTTGGCATTCTTT
  TCCCATTGACTATATACATCTCTATTTCTCAAATGTTCATGGAACTAGCTCTTTTATTTTCCTGCTGGT
  TTCTTCAGTAATGAGTTAAATAAAACATTGACACATACAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000132.4
• Summary: This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]
• Locus ID: 2157
• MW: 71.4