PAPOLG (NM_022894) Human 3' UTR Clone

CAT#: SC216566

3' UTR clone of poly(A) polymerase gamma (PAPOLG) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PAPOLG
• ACCN: NM_022894
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC216566 3’UTR clone of NM_022894
  The sequence shown below is from the reference sequence of NM_022894. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AATTCCATTCGACTGACCCTTAATCGGTAAAAGCAGTGCCTCCTCACTTAAGTGAACAAGCAATCATTT
  AGTGGCATAGATGCAGCCACTTGTTTTTTAAATAGAAGTGGCTGTCATACGTGAAATAAGGTGAAAGTG
  ACAGCCTTCAGTCTAATAACCTTGAAGTGGTTTTTGAACTGTCAAACTTTGACCTGTAGATGCTGTAGC
  ATTCTCTCACTGATTGCTACATACACTTCTCTGAGGATCACCTGCTGTCAAATTGCCATCTACAGTATT
  ACAGCTTTGCATTTGGGGGTTTTACTGCCTTAACTTTCAATGCTTGTTATGGGAACCAGTTCTTAGCCA
  CTTGGACACTGATAACAAGTCCTGAACTCCTTTTTTGTTTTGTTTTTTGTGTTTTTCTTTTTTTGTCTT
  ATGTTGTAATCATTGTATAGAGCTAAAAAAATTGAAAACAAACAAAAAAATTGTCTTGTATTTTCCAAA
  TGTTAATACATTTACTTTAGCATTGAAGCCACTTTGAAACCTGAGATAAATGAATGTGAGGTATCTTTT
  CTGCTTTCCTCATTTGTGTAGATGTACTGTCTGTTCTGTTGATTTAAATTATTTTTTTCCAGATTGGCA
  CATGAAATATTTAAACTTTTTTGTGTGCCTTTCTGTCCAAATGTTGCATAGTTACCAGGGAAGATTAGT
  CCAGTGATTACATAAGAGTTGGGCACCATAAATTCTCTATATTTTGCCTCCCATGGAGGCCTTTGAAAT
  GCATCTTTATTAAAAATCAAAATATAACCAGGATACTGAAAGTCAGTATATGAATGGTAAAATTGTTAC
  ATATCCTATTTCATGCCATTCTTGTTAGTTGACTGGTATTTTTTACTGAGGAGCAACTCATTCCAGCAT
  CAACAATAAGATAACCTTTAAGTATGGCACACTTGTATTTTTGAGGTGTAAAATTAACTTGGCATGATA
  ATTCCTGATCATTATTTACCCCACAACTTCAAATAGTTTCTTCACGGACTAGGCATGCAGAAATAAGCA
  GTGGATTTTATTGAAACCTAAAGGCATTTTGAATGACATTGTTACCAACCATTAATTGGCTCAGGACCT
  TTGTAATTTTTATTTAACTATATGAGTTGTCTTTTTTTACGCTGCTTTTTTCAATGCATTTCTTAATAT
  TTTTTAAGTTTCATGAATGCATGCTCAGTTTATTAAAATCCATAACCATGTAATTCTTGTAATATGTTG
  ATTCAGTGTTTTGTAAATGAAGTCGTATGTATTTTCAGAGTATTTTTGTATGTACTGTAAGATACCATC
  TTTTCAAAGAGAAACGTTTAAAACCTTTATTGTCTCTCCTTAGTCTAATTTTTTAAATATGGATTATGC
  TTGAATTACTATATTTAAAATGAAAATGTATAGATTACACAGCCAGGAATGCTAGTATCTACCACCTTC
  TTTAGATTTCACTCAGCATTTAGTAGGTCCAGTAGGAAAAACCCAAAATGGTACATTGAGTAGTGTGGG
  GAAGACACCTCGTGTATTATAGGACTCACGGGATGCCATCTAGCAAGTGGAGAAATAGCAGTCCATTTT
  CTCTTGCAAGCCCTTTACCATTGAGTTGAAATTCTTGAATTGCCAGAGAGCCGTATCCTCCAGGTCCCA
  CTCCTGTTCATTGCCCCTTAGGTCTCCAAAATGTAAATTCTAATGTTTACCTTGTACCATGGAAAACAT
  GAAAAGAGTCTTAGAAGTAAAGAACAACAAGGAAAAAAATGTTTGTTCTATTATTCCCTTTTTATATAG
  CAGCAAAAGATAAGAATAAAATCACATAACCAAGTTGAAGCTTCCTTGGCAAAGCTGTTGTCTTAGCTG
  ACTCTTTCCCTTTATTCAGATGTTTTGGGATTGTTCTACAAATAGTCTATTTGAGCAAAAATGAATGAT
  TATGAAAGAACCGAAGAATTATCACCTGACTTCAACTTTTGAAAGCTTTAGTAAGGAATTTGATTAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_022894.4
• Summary: This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14. [provided by RefSeq, Jul 2008]
• Locus ID: 64895
• MW: 77