PAPOLG (NM_022894) Human 3' UTR Clone
CAT#: SC216566
3' UTR clone of poly(A) polymerase gamma (PAPOLG) for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | PAPOLG |
ACCN | NM_022894 |
Insert Size | 2000 bp |
Sequence Data |
>SC216566 3’UTR clone of NM_022894
The sequence shown below is from the reference sequence of NM_022894. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC AATTCCATTCGACTGACCCTTAATCGGTAAAAGCAGTGCCTCCTCACTTAAGTGAACAAGCAATCATTT AGTGGCATAGATGCAGCCACTTGTTTTTTAAATAGAAGTGGCTGTCATACGTGAAATAAGGTGAAAGTG ACAGCCTTCAGTCTAATAACCTTGAAGTGGTTTTTGAACTGTCAAACTTTGACCTGTAGATGCTGTAGC ATTCTCTCACTGATTGCTACATACACTTCTCTGAGGATCACCTGCTGTCAAATTGCCATCTACAGTATT ACAGCTTTGCATTTGGGGGTTTTACTGCCTTAACTTTCAATGCTTGTTATGGGAACCAGTTCTTAGCCA CTTGGACACTGATAACAAGTCCTGAACTCCTTTTTTGTTTTGTTTTTTGTGTTTTTCTTTTTTTGTCTT ATGTTGTAATCATTGTATAGAGCTAAAAAAATTGAAAACAAACAAAAAAATTGTCTTGTATTTTCCAAA TGTTAATACATTTACTTTAGCATTGAAGCCACTTTGAAACCTGAGATAAATGAATGTGAGGTATCTTTT CTGCTTTCCTCATTTGTGTAGATGTACTGTCTGTTCTGTTGATTTAAATTATTTTTTTCCAGATTGGCA CATGAAATATTTAAACTTTTTTGTGTGCCTTTCTGTCCAAATGTTGCATAGTTACCAGGGAAGATTAGT CCAGTGATTACATAAGAGTTGGGCACCATAAATTCTCTATATTTTGCCTCCCATGGAGGCCTTTGAAAT GCATCTTTATTAAAAATCAAAATATAACCAGGATACTGAAAGTCAGTATATGAATGGTAAAATTGTTAC ATATCCTATTTCATGCCATTCTTGTTAGTTGACTGGTATTTTTTACTGAGGAGCAACTCATTCCAGCAT CAACAATAAGATAACCTTTAAGTATGGCACACTTGTATTTTTGAGGTGTAAAATTAACTTGGCATGATA ATTCCTGATCATTATTTACCCCACAACTTCAAATAGTTTCTTCACGGACTAGGCATGCAGAAATAAGCA GTGGATTTTATTGAAACCTAAAGGCATTTTGAATGACATTGTTACCAACCATTAATTGGCTCAGGACCT TTGTAATTTTTATTTAACTATATGAGTTGTCTTTTTTTACGCTGCTTTTTTCAATGCATTTCTTAATAT TTTTTAAGTTTCATGAATGCATGCTCAGTTTATTAAAATCCATAACCATGTAATTCTTGTAATATGTTG ATTCAGTGTTTTGTAAATGAAGTCGTATGTATTTTCAGAGTATTTTTGTATGTACTGTAAGATACCATC TTTTCAAAGAGAAACGTTTAAAACCTTTATTGTCTCTCCTTAGTCTAATTTTTTAAATATGGATTATGC TTGAATTACTATATTTAAAATGAAAATGTATAGATTACACAGCCAGGAATGCTAGTATCTACCACCTTC TTTAGATTTCACTCAGCATTTAGTAGGTCCAGTAGGAAAAACCCAAAATGGTACATTGAGTAGTGTGGG GAAGACACCTCGTGTATTATAGGACTCACGGGATGCCATCTAGCAAGTGGAGAAATAGCAGTCCATTTT CTCTTGCAAGCCCTTTACCATTGAGTTGAAATTCTTGAATTGCCAGAGAGCCGTATCCTCCAGGTCCCA CTCCTGTTCATTGCCCCTTAGGTCTCCAAAATGTAAATTCTAATGTTTACCTTGTACCATGGAAAACAT GAAAAGAGTCTTAGAAGTAAAGAACAACAAGGAAAAAAATGTTTGTTCTATTATTCCCTTTTTATATAG CAGCAAAAGATAAGAATAAAATCACATAACCAAGTTGAAGCTTCCTTGGCAAAGCTGTTGTCTTAGCTG ACTCTTTCCCTTTATTCAGATGTTTTGGGATTGTTCTACAAATAGTCTATTTGAGCAAAAATGAATGAT TATGAAAGAACCGAAGAATTATCACCTGACTTCAACTTTTGAAAGCTTTAGTAAGGAATTTGATTAAA ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_022894.4 |
Summary | This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14. [provided by RefSeq, Jul 2008] |
Locus ID | 64895 |
MW | 77 |
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