SCD5 (NM_001037582) Human 3' UTR Clone

CAT#: SC216484

3' UTR clone of stearoyl-CoA desaturase 5 (SCD5) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SCD5
• Synonyms: ACOD4; FADS4; HSCD5; SCD2; SCD4
• ACCN: NM_001037582
• Insert Size: 1818 bp
• Sequence Data:
  Insert Sequence

  >SC216484 3’UTR clone of NM_001037582
  The sequence shown below is from the reference sequence of NM_001037582. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAGGCCAGGACTGGAGACAGCAGTGCTTGAACTTGGAACAGCCATCCCACATGTCTGCCGTTGCAACCT
  CGGTTCATGGCTTTGGTTACAATAGCTCTCTTGTACATTGGATCGTGGGAGGGGGCAGAGGGTGGGGAA
  GGAACGAGTCAATGTGGTTTGGGAATGTTTTTGTTTATCTCAAAATAATGTTGAAATACAATTATCAAT
  GAAAAAACTTTCGTTTTTTTTTTTGTTTGTTTTGTTTTTGAGACAGAGTCTCACTCTGTCACCCAGGCT
  GGAGTGCAGTGGCGCAGTCTCGGCTCACTGCAGCCTCCACCTACCTGGTTCAAGCAATTCTCCTGCCTC
  AGCCTCCTGAGTAGCTGAGATTACAGGAGCCTGCCACCACACCCAGCTAATTTTTTTGTATTTTTAGTA
  GAGACAGGGTTTCATCATGTTGGCCAGACTGGCCTCGAATTCCTGACCTCAGGCAATCCACCCGCCTCG
  GCCTCCCAAAGAGCTGGGATTACAGGCGTGAGCCACCGCACCCTGCCGAAAAAAACTTTTTTTTTTTTG
  AGACGGAGGCTCGCTCTGTCCCCCAGGCTGGAGTGCAGTGGCGAGATCTCAGCTCACTGCAAGCTCCGC
  CTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGAGCCAGCGCGCCCAGCCTAAA
  AAACTTTTCAAGTCAATATTACTACGATTTAACATTAGAGTGTGGACATGTGATTTAATCGCTATAGCT
  AAAATACGTCAAATATACGTTGTCATGTGCTTGAACATGATGCTAACCCTGACAGGATGAAGGAAAGTA
  ATATTCTTTCAGTGTAGTTCAGGAGAGCATTTGTTTTCTTTTCTACCAATTAACCCATCATTGCTTTTA
  AACAACCATCTGAAGGAGCAGAGAGGCAGGGTAGAAGACAGAAGGGGGATCTATGTGGTAACTAAAGAA
  TGTTTCTGTTTTGTTAATTATTGTGTGTGTGTGGTTTTATTGTTTGCTTAAGAGAATCAAAAACTGAAA
  AAAATGAGAATACAGGAAATGGCTCTTGTTTATTTTTTTGCTGTGTTTACAGCTTGTTAATGCTCTACT
  GTCTTTGTTTCAAGAGAGATTTGTTCACTGCCCAGCTCGTTTTGTGTCCTGAGCCCTATGGCCAGCCCA
  CCTTATAAATCATGCCTGTTTAGATGTTTGATTTTGTTCTGTTTGCTATTGTTATCTTAAAGGTGTATA
  ACTCTGACATGCCAGACATCAAATTAAGCTCAAATTAAGCTCTCGTTTAAATGTTTAAGCACCTAATTT
  ATATTCTAATTGATCCCAGCCACTGATGCATGTACTTTAGCTACTTCTGCTAAATAAGCATATTAATTT
  TCCACATCAGACCATCAGATCTTGAGAACCAACAGTTATCTAGAATTCCGTGTCTACTAATGTTTCACC
  TGCATGCAGCCTTCATTAATTTTGTAGCAAAATATAAAGTGATCATTATGTAGCTTCTGGATTAAAAAA
  ATTTGTGTGTGAAGTTGCTTTGTAAAGTGCATGTGGAATTAATGGGACAGTGTGCCCTTTGTGTTAGAT
  GTTAGAGCAAAAGAAAGGGCTTATAGTGTTAGTATTGGAGCACTTTGAAGATAGATATTTTCAGAAAAG
  ATGTAGGATTTAAAAGTTAAATTTTAAATTTTAGAAAAAGATATGATGGCAATTGGAAATAGTCACAAT
  GAAGTTCTTCATCCAGTAGGTGTTTAACAGTGTTATTTTGCCACTGGTAATGTGTAAACTGTGAGTGAT
  TTACAATAAATGATTATGAATTCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001037582.3
• Summary: Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an integral membrane protein of the endoplasmic reticulum that catalyzes the formation of monounsaturated fatty acids from saturated fatty acids. SCD may be a key regulator of energy metabolism with a role in obesity and dislipidemia. Four SCD isoforms, Scd1 through Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 (MIM 604031) and SCD5, have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms, as well as with rat Scd1 and Scd2. In contrast, SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates (Wang et al., 2005 [PubMed 15907797]).[supplied by OMIM, Mar 2008]
• Locus ID: 79966
• MW: 68.4