CHMP2B (NM_014043) Human 3' UTR Clone

CAT#: SC216252

3' UTR clone of chromatin modifying protein 2B (CHMP2B) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CHMP2B
• Synonyms: ALS17; CHMP2.5; DMT1; FTDALS7; VPS2-2; VPS2B
• ACCN: NM_014043
• Insert Size: 1764 bp
• Sequence Data:
  Insert Sequence

  >SC216252 3’UTR clone of NM_014043
  The sequence shown below is from the reference sequence of NM_014043. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CGGCAACTCAAGGCTTTAGGAGTAGATTAGTCAAAAGAAGTCATACTATTTTGCTTACTTATAATTATG
  TAGTATAAACCAAGCACAGTGCAGATTTCTTTTACAAAACACATGTATTTTGCAAAAAAAAAAAAAATG
  AAGACCATGAGTGAACAGTTGTTTCCTAACCCATGGCTATTTAGAATCTTTTGCCAAAGAATGACAATG
  ATGCAAAAATGGGAACAGTTTGGATTTTAATTAGAACTGTTTAGGAGTGATGATGTGTAAAAAGTTGAC
  TTCTCTTTTGCATGGCACAGAGAAATTATATTCCTTACTTCATGTCAGTTTATGTTCTAAATCTTTTTC
  ACTGAATATAAAAATCTTGTTAAATGCCATTAGGCACCAACTTAAAGAGGGTTGTAAAAATATTAAAAG
  TATATCGTTAATTCTGTATCTGTTGCTTGTCTTTTGTAAGTGATTATGTGTTATGACCATAGGTGGTTA
  CAGCTGCCAAATTATTTTTAAATGGTCAAAAAGAAGAGTGCTATTTAAACATCTGTCTTAAACAAAAAC
  TGTCATAACTTTTCTTTTTTCTTTTTCCATTAGGAGAACATTCTAGTTGGTAAATTTCAAAATGTGCTT
  GACACCTGCCTTAAATAGCACAGACCTATTGTGCACATCTTTAAATTATTTCAGCTGGCAGAAAAGAAT
  TACATTTAAAACTGAAATCAAGGCCTCAATACAAAGATTATCCTGGCTCTTTTCTATCTCTGTGGGCCT
  AATTGAAATATGTACTCTTATTTTAGACACGCCTCTGTTAAAACAGACCAGGTTTTCCTGGTCTCAGAC
  CTATGATGACTTGTCCCTTTGATGTCACTACTGTGAATTGAATATAATTAGTAAAAATAGACGATGAAT
  AAATAACACTTTATAGTAAGAAAACAATATATTTTGGCCATCTAAAAATGAGAATTATAATTATATGAA
  TTATAATTTAAACTGTTTAATTTTGTTTAATGTGTATATTGAATCTTCCAAATTGAAGCCATTATTCTC
  AATTAAGTACTACAACTATGACAATGCTTGACCTACATTTCTAAAATAAAAATTCACATTTTTTGATAA
  ATAAACTACAGTTTTACCAGAAATTACTATCTAAATGTGTATTAGCAGTATTTTTTAAGGTGAAATTGC
  CTTGGTATCTAATGAATGTGTAGACAGGGAGATAAAATGAAGGATTGCCAGACTAGTTAGAATAGAATT
  TAGGATTAGGTTAGTTTTGAAAAATGATGTTGTAATATATGGGTTCTAACACATCCTACCATAAAAACT
  GGAGGAGATATGTGTAACCTGGTTAATTTGGGATGGTGGACATTTTGGGCTAATACTGACAAAATACAT
  CTTAGGACTAGTATACATGTGACACGGATTGCTAGGAGGAATGAAAAACTAAACTGTATAGTTTATATT
  CCGTAAACCATTTTATAATGTTCAAAGATTAGGTTTTGTTATTGATAGTATTAAATACACAGTTTCTCT
  TAACAGTGATGGGTGAAAACATTTTACCGGATTATGGAATGTTTACCAGAACATGTTTTGATTCTTGAA
  TGTACATAATAATGCCATCTAACTTATTTACGTTCTTGTTTACATGTGGGAGCTTTTGTTTTCAAAAAT
  TATTTTGTTAAAAAATCTCAATAAAGATTTATTATTGTTGTTCTTTTCTTACCTTTTTTGCTCTTTTTG
  GTTCCTGCTAAAATTAAAAATTTTATGCATATTTGGTAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_014043.4
• Summary: This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration. [provided by RefSeq, Jul 2008]
• Locus ID: 25978
• MW: 70.3