HNF 4 alpha (HNF4A) (NM_178849) Human 3' UTR Clone

CAT#: SC216183

3' UTR clone of hepatocyte nuclear factor 4 alpha (HNF4A) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HNF 4 alpha
• Synonyms: FRTS4; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF-14; TCF14
• ACCN: NM_178849
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC216183 3’UTR clone of NM_178849
  The sequence shown below is from the reference sequence of NM_178849. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCGACCATCACCAAGCAGGAAGTTATCTAGCAAGCCGCTGGGGCTTGGGGGCTCCACTGGCTCCCCCCA
  GCCCCCTAAGAGAGCACCTGGTGATCACGTGGTCACGGCAAAGGAAGACGTGATGCCAGGACCAGTCCC
  AGAGCAGGAATGGGAAGGATGAAGGGCCCGAGAACATGGCCTAAGGGCCACATCCCACTGCCACCCTTG
  ACGCCCTGCTCTGGATAACAAGACTTTGACTTGGGGAGACCTCTACTGCCTTGGACAACTTTTCTCATG
  TTGAAGCCACTGCCTTCACCTTCACCTTCATCCATGTCCAACCCCCGACTTCATCCCAAAGGACAGCCG
  CCTGGAGATGACTTGAGGCCTTACTTAAACCCAGCTCCCTTCTTCCCTAGCCTGGTGCTTCTCCTCTCC
  TAGCCCCTGTCATGGTGTCCAGACAGAGCCCTGTGAGGCTGGGTCCAATTGTGGCACTTGGGGCACCTT
  GCTCCTCCTTCTGCTGCTGCCCCCACCTCTGCTGCCTCCCTCTGCTGTCACCTTGCTCAGCCATCCCGT
  CTTCTCCAACACCACCTCTCCAGAGGCCAAGGAGGCCTTGGAAACGATTCCCCCAGTCATTCTGGGAAC
  ATGTTGTAAGCACTGACTGGGACCAGGCACCAGGCAGGGTCTAGAAGGCTGTGGTGAGGGAAGACGCCT
  TTCTCCTCCAACCCAACCTCATCCTCCTTCTTCAGGGACTTGGGTGGGTACTTGGGTGAGGATCCCTGA
  AGGCCTTCAACCCGAGAAAACAAACCCAGGTTGGCGACTGCAACAGGAACTTGGAGTGGAGAGGAAAAG
  CATCAGAAAGAGGCAGACCATCCACCAGGCCTTTGAGAAAGGGTAGAATTCTGGCTGGTAGAGCAGGTG
  AGATGGGACATTCCAAAGAACAGCCTGAGCCAAGGCCTAGTGGTAGTAAGAATCTAGCAAGAATTGAGG
  AAGAATGGTGTGGGAGAGGGATGATGAAGAGAGAGAGGGCCTGCTGGAGAGCATAGGGTCTGGAACACC
  AGGCTGAGGTCCTGATCAGCTTCAAGGAGTATGCAGGGAGCTGGGCTTCCAGAAAATGAACACAGCAGT
  TCTGCAGAGGACGGGAGGCTGGAAGCTGGGAGGTCAGGTGGGGTGGATGATATAATGCGGGTGAGAGTA
  ATGAGGCTTGGGGCTGGAGAGGACAAGATGGGTAAACCCTCACATCAGAGTGACATCCAGGAGGAATAA
  GCTCCCAGGGCCTGTCTCAAGCTCTTCCTTACTCCCAGGCACTGTCTTAAGGCATCTGACATGCATCAT
  CTCATTTAATCCTCCCTTCCTCCCTATTAACCTAGAGATTGTTTTTGTTTTTTATTCTCCTCCTCCCTC
  CCCGCCCTCACCCGCCCCACTCCCTCCTAACCTAGAGATTGTTACAGAAGCTGAAATTGCGTTCTAAGA
  GGTGAAGTGATTTTTTTTCTGAAACTCACACAACTAGGAAGTGGCTGAGTCAGGACTTGAACCCAGGTC
  TCCCTGGATCAGAACAGGAGCTCTTAACTACAGTGGCTGAATAGCTTCTCCAAAGGCTCCCTGTGTTCT
  CACCGTGATCAAGTTGAGGGGCTTCCGGCTCCCTTCTACAGCCTCAGAAACCAGACTCGTTCTTCTGGG
  AACCCTGCCCACTCCCAGGACCAAGATTGGCCTGAGGCTGCACTAAAATTCACTTAGGGTCGAGCATCC
  TGTTTGCTGATAAATATTAAGGAGAATTCATGACTCTTGACAGCTTTTCTCTCTTCACTCCCCAAGTCA
  AGGGGAGGGGTGGCAGGGGTCTGTTTCCTGGAAGTCAGGCTCATCTGGCCTGTTGGCATGGGGGTGGGA
  CAGTGTGCACAGTGTGGGGGCAGGGGAGGGCTAAGCAGGCCTGGGTTTGAGGGCTGCTCCGGAGACCGT
  CACTCCAGGTGCATTCTGGAAGCATTAGACCCCAGGATGGAGCGACCAGCATGTCATCCATGTGGAAT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_178849.3
• Summary: The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]
• Locus ID: 3172
• MW: 73.1