MMD (NM_012329) Human 3' UTR Clone

CAT#: SC216169

3' UTR clone of monocyte to macrophage differentiation-associated (MMD) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MMD
• Synonyms: MMA; MMD1; PAQR11
• ACCN: NM_012329
• Insert Size: 1742 bp
• Sequence Data:
  Insert Sequence

  >SC216169 3’UTR clone of NM_012329
  The sequence shown below is from the reference sequence of NM_012329. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGTCCTACGGACTTTATGCGGCATTTATGACCAATCTGTACTAATTCTCCAAACCAGTATTATTTCAAT
  TATGGCACTTGGGAGTGGGGTGAGAGCTAAACATTGCACAGGGAAAGAAAAAAAATAACTGCACTGACT
  TTATATCTTTTGAATATAATTACTGTGAAAGTATAAAGGCTGTGTTCTGGAATTTTCTGCCTCACAGCA
  AATAAATAAGGTAGTGAATTAATTATTCATTCCATTCCACTATCATGAAGGACTCTGAATAGACTTGGC
  CAACTGATGTTTACAAACCAGACTTTTATATTTTAATTTTACAGATTTTACTACATGATTTTTCTAAAT
  TACTATGTCAGGTTGTAAAAGTCAGTGCAATAACAAACCTTCCTTTTTAAGAAGAAAATTGTTTCTATT
  ACTTTCCCATTCACTAGGTAAAGAATCATGGACAGAACTTACACTACTTTTTACCATGTTTCATCTTGG
  CATAACATGGTTCTTTTTTAAATAGAAACTTTAGTTTTTTGTAAATTTTTAAAAAAATATTTCATTGAT
  ATGCATCTCTGCAGGTCCTCATTCATGTTGTAAATTTTTGCAGCAAGCAGTCAACATTCCACAAACGAA
  CAAACATTATACCTCTTCTGATAGTTTTATTAAGCATGGAGAAATTGCCAATTTTTAAAAACTGCAGTT
  TTCCAAACTTTTCTGCCAACCTCTTACTCTGAATTCAGTGCTGCTTTGGGACATATACTTGACCTAGCT
  TGGTTTACCAGTGATGGAAAAGTATTTTGATATCATTAACTTTTTCAAAAGATCCAACTTTTTCTCTAT
  GCCTTTGCCACATTCTCTTCAGGGTCTCTTTCCACAGTGGATAAATGTTTTTTCTGTATTATGACAGTA
  TTGTTGTGATGGCCATCTGCTGGAAACTCCTGAAGAGCATTATGTATTACAGTGAGCAGTTGTATTGCC
  TGTTTGGTGCCCAATGGTTAAGTCATTGTCACTTAGCTTTATATTGTCAGTTTGATATTTATTTTAAAT
  TGTGGAACTAGATGCATAAATTCACATTTCTGCCTTTCCTTTGCATCTTCTCATATATTGTGTTTTTTT
  TTTTTTTTCCTAGAAAAAATATTTAAAGCATTGTTTGACAGGTAGAAACTCATGTATCTGTAGTCCATG
  AGTTATATCCTGGCTCAGTGGAGTGATATTTATGTATTATTTTTACTTTTCTCTCAGTGTCTTATATTA
  AGATTAACATGTTGTTAATAGTTGCTTTGTTGATTAATCTCTCTTGTTGGTGTTTTAATAAATGAAATA
  GGCTTGCCTTTAGATCGGGTGCTGATATTGCCTGTTTCCTAGTAATGGGCTGATCAAATGATCAGTGGA
  ATTCTTGGTTTGATGATAACCTTATTAATTGAAATTTTTTACTGATGTGGCTTTAAAAGAGGTTTATTT
  TGTATATGTTTAGAACTCTCTGATTTTGATGAATTATATGGGAATGAGAAACAGAAGAAGTGGTATTTG
  CTGGCGAGTTAAATAGGCAAGGTACCCAGTGATAACACCAACCAAACCACTCCTATCTGCATGATTCTG
  AACATCTGGATGCCTGTTGTTTTACTGTGTATATTTTATTTTTAATATATTAACTTTGTGGATTCATTT
  AAGGTCTACTCAAAAGTAACACTGTCAAAACCACTAATATGTATGTAAAAATTGTGCTGTATACTACAA
  TAAAGTTGTTACTTGGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_012329.3
• Summary: This protein is expressed by in vitro differentiated macrophages but not freshly isolated monocytes. Although sequence analysis identifies seven potential transmembrane domains, this protein has little homology to G-protein receptors and it has not been positively identified as a receptor. A suggested alternative function is that of an ion channel protein in maturing macrophages. [provided by RefSeq, Jul 2008]
• Locus ID: 23531
• MW: 67.6