GGA3 (NM_014001) Human 3' UTR Clone

CAT#: SC216129

3' UTR clone of golgi associated gamma adaptin ear containing ARF binding protein 3 (GGA3) transcript variant short for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GGA3
• ACCN: NM_014001
• Insert Size: 1710 bp
• Sequence Data:
  Insert Sequence

  >SC216129 3’UTR clone of NM_014001
  The sequence shown below is from the reference sequence of NM_014001. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCTCCTGTGGAACAGTGGGGGAACCTATGACCCCAGAGGACGCTGTGATCTCCACCTTGAAGCCAGGCA
  GGCTGCCCTGAGACGAGGAGGGCTCTGGTCCCGTCACTCTCCATGCCCTGACAACAGTGCTTGTAGCTT
  TGATGTGGAACAGGGGCCCTGGGCATTTCTGCTGAGAACCAAACTGCTGCTGTGATAACCTCTCCTTTG
  GCCCCTAAAAGGACCTGTTTTTATCTACCTGTGTGACTTGAAGTGGCCATATGCTGTCAGGGGTGCGGA
  GTGGCCCCTGACTTCACTGCTGTCCCGGGAACATGGACCCCCAGGCTTGGCGTAGGTGTTTGCTTCCTT
  CTACTGGCATTCACTGAAGCCACTGGGGTGGGGGGTGGGGGGTGGGAGTCTCTAAAGAGAGACTGTCAT
  GGGTCATTCCCCACAAGAGCCACATCCTCACACCTGACAGATGCACGGCCCAAGGGGCTGCAGCCCTGT
  TGCAATTCCATGCTTCCCCCGCCAACCAGCTCCTGCTGCCATCCCCAGGGAGGTGGCCCAGGAAGGTGC
  CTGGCCCAGAATAAGGAACTGGCATACTGCAAAGTCCCCAGCCCTGCCTCTGGTGGACAGCATCGTCCT
  GGAATGGCCACGGAGTGATGAGTTGTGTGCTTGTCCCTGGCAGTGGCAGGCTGTGTCCTATGGACATCT
  TGGCAGGACATGGAATTTGGCCTCATGACAGGCCCAACTAGGGATAGGAAGGAAAATGAAGAGAGCCAG
  TATTTCCCCTTCTCCAGAAGCAGGTACTCAGCTTTCTGGGAAAAGCGTGCCTCCAGCCGTGGGGACAGG
  CCATCCTACTGACTACCTCTTGCTTGGCATGAAATAAACTGCTATCCTCCCCTTGGAATCTACCGCCAC
  TCTACATCCTACTGCTTTGGCCTCCCTCTCCTCTCACCAGATGGCATGTGGTGTGGCACCTGTGGCTGG
  ACACAGGAGGCCTCAGGATCACAAATGTTACACTAGACATATGTCCTAATGTGCTGCCCAGAAACCTCA
  ACTGTTCCCCAGCTACTGAGGGGCACTGTCAGCGAGATGTTGGGTCTGGAGGTGATGAGATCGGGCCAC
  ACTTGAGCTGAGTCACCAGACCCTATTGCTTCAACAGTGCTTGGCCCCCGCCAGCTTGTCCCAGCCACT
  CTAGCTGCTGGATGTGATCCTGGGACATGTACTCCAAGCCTCCGTCACAAAAAAAAAATCACCAGCTGC
  CATAGACACGGGGGAAGCTTGCGGAGCCCAGGTGAACAAGCTCAGCAATCGGACATCTCTGGGGAAAGG
  AAGGTGGCACAGACCATGTTCCCTGGTTCCTCCCTGCCCCTTGCCAGGCTTCCTTATTCCTTACTATGG
  GAAGAGGTCATATCCCTTCCCTGCCCCTCGCTGTCTTTAGCAAGCAGGTTTCACTGCTTCATTAGAAGA
  GGACAAGTCAAAAGTGAATCATTTTTCACTACTTAAGGAATAAATCCAAGAGCTTTCCAGAGACTGGCT
  GCTGCAGCCCTGGGAATGTCTGTGGAATTACTATGTGGAAATGGAACTTTGTGTTATGCTCTAGACATT
  ACAGTTATTTGAGTGTTACTCGTTACTGTTGAGGTCAGTGCTTCGTGGCAAATGGCTGTACTGGATATC
  CCAGCTCTGCTGCCCTTGTTTTGCTGCATGTTAAATAAAACCATTTTCACTGTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_014001.5
• Summary: This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010]
• Locus ID: 23163
• MW: 61.5