Factor VII (F7) (NM_019616) Human 3' UTR Clone

CAT#: SC216012

3' UTR clone of coagulation factor VII (serum prothrombin conversion accelerator) (F7) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: F7
• Synonyms: SPCA
• ACCN: NM_019616
• Insert Size: 1707 bp
• Sequence Data:
  Insert Sequence

  >SC216012 3’UTR clone of NM_019616
  The sequence shown below is from the reference sequence of NM_019616. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGAGTCCTCCTGCGAGCCCCATTTCCCTAGCCCAGCAGCCCTGGCCTGTGGAGAGAAAGCCAAGGCTGC
  GTCGAACTGTCCTGGCACCAAATCCCATATATTCTTCTGCAGTTAATGGGGTAGAGGAGGGCATGGGAG
  GGAGGGAGAGGTGGGGAGGGAGACAGAGACAGAAACAGAGAGAGACAGAGACAGAGAGAGACTGAGGGA
  GAGACTCTGAGGACATGGAGAGAGACTCAAAGAGACTCCAAGATTCAAAGAGACTAATAGAGACACAGA
  GATGGAATAGAAAAGATGAGAGGCAGAGGCAGACAGGCGCTGGACAGAGGGGCAGGGGAGTGCCAAGGT
  TGTCCTGGAGGCAGACAGCCCAGCTGAGCCTCCTTACCTCCCTTCAGCCAAGCCCACCTGCACGTGATC
  TGCTGGCCTCAGGCTGCTGCTCTGCCTTCATTGCTGGAGACAGTAGAGGCATGAACACACATGGATGCA
  CACACACACACGCCAATGCACACACACAGAGATATGCACACACACGGATGCACACACAGATGGTCACAC
  AGAGATACGCAAACACACCGATGCACACGCACATAGAGATATGCACACACAGATGCACACACAGATATA
  CACATGGATGCACGCACATGCCAATGCACGCACACATCAGTGCACACGGATGCACAGAGATATGCACAC
  ACCGATGTGCGCACACACAGATATGCACACACATGGATGAGCACACACACACCAATGCGCACACACACC
  GATGTACACACACAGATGCACACACAGATGCACACACACCGATGCTGACTCCATGTGTGCTGTCCTCTG
  AAGGCGGTTGTTTAGCTCTCACTTTTCTGGTTCTTATCCATTATCATCTTCACTTCAGACAATTCAGAA
  GCATCACCATGCATGGTGGCGAATGCCCCCAAACTCTCCCCCAAATGTATTTCTCCCTTCGCTGGGTGC
  CGGGCTGCACAGACTATTCCCCACCTGCTTCCCAGCTTCACAATAAACGGCTGCGTCTCCTCCGCACAC
  CTGTGGTGCCTGCCACCCACTGGGTTGCCCATGATTCATTTTTGGAGCCCCCGGTGCTCATCCTCTGAG
  ATGCTCTTTTCTTTCACAATTTTCAACATCACTGAAATGAACCCTCACATGGAAGCTATTTTTTAAAAA
  CAAAAGCTGTTTGATAGATGTTTGAGGCTGTAGCTCCCAGGATCCTGTGGAATTGGATGTTCTCTCCCT
  GCCACAGCCCTTGTCAATGATATTTCACAGAGACCCTGGGAGCACCTGCTCAAGAGTCAGGGACACACG
  CATCACTAAATGCAAGTTCCCAGGCCCTGGCTGCAGTGGGAGGACCTGGCAAGCTGCACTCTTGCTGAG
  TCCCCAGGGTGGTGGAAGAAGAATGAGAAACACATGAACAGAGAAATGGGGAGGTGACAAACAGTGCCC
  CCACTCAGACTCCGGCAAGCACGGCTCAGAGAGTGGACTCGATGCCATCCCTGCAGGGCCGTCCTGGGC
  ACCACTGGCACTCACAGCAGCAAGGTGGGCACCATTGGCACTCACAGCAGCAAGGCAGGCACCAGCAAC
  CCACCTCGGGGGCACTCAGGCATCATCTACTTCAGAGCAGACAGGGTCTATGAACTACAGCCGTGGGCT
  GCTTCCAAGGCACCCTGCTCTTGTAAATAAAGTTTTATGGGAACACACCCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_019616.4
• Summary: This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015]
• Locus ID: 2155
• MW: 64.8