Plakophilin 2 (PKP2) (NM_004572) Human 3' UTR Clone

CAT#: SC215949

3' UTR clone of plakophilin 2 (PKP2) transcript variant 2b for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: Plakophilin 2
• Synonyms: ARVD9
• ACCN: NM_004572
• Insert Size: 1699 bp
• Sequence Data:
  Insert Sequence

  >SC215949 3’UTR clone of NM_004572
  The sequence shown below is from the reference sequence of NM_004572. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GCCAAAGCCTACCACTCCCTTAAAGACTGAGGAAAATGACAAAGTATTCTCGGCTGCAAAAATCCCCAA
  AGGAAAACACCTATTTTTCTACTACCCAGCCCAAGAAACCTCAAAAGCATGCCTTGTTTCTATCCTTCT
  CTATTTCCGTGGTCCCCTGAATCCAGAAAACAAATAGAACATAATTTTATGAGTCTTCCAGAAGACCTT
  TGCAAGTTTGCCACCAGTAGATACCGGCCACAGGCTCGACAAATAGTGGTCTTTGTTATTAGGGCTTAT
  GGTACATGGCTTCCTGGAATCAAAATGTGAATTCATGTGGAAGGGACATTAATCCAATAAATAAGGAAA
  GAAGCTGTTGCATTACTGGGATTTTAAAAGTTTGATTTACATTTATATTCCTTTTCTGGTTCCCATGTT
  TTGTCACTCATGTGCACATTGCTTCGCCATTGGGCCTCCAGTGTATTGTTCTGCAGTGTTGAAACAGAA
  TGGAAATGACAAGAAATATCTGCAGTTATCCAGGAGAAAGTATAATGGCAAAATTATTGGTTTCTTTCT
  TTACTTTGTGCTTGTTTTTATCCCCTTGGGTTGTTTTTCTCTGATTTTTAAATAAACTTAAGAAATTTA
  GATTACAGAGTATGCATGACTGTAAGAAAAAGAAATTGAGAGGAAGTGATCATAGCAAATTAAAGAAGT
  CTTTTCCTCCCAGAACTTAAAGTAAAATAAAAAATAAATAAATAAATAAAATCTTTTCCACAGAGAAAG
  GCAACTGTGATGATAAAATTTAACGTTCCCCCAAACACTGAGTCAATGAGATTTTTCTCAGGAGATACT
  TTACCTATAACAACGCCGTTAAATCCAAATCTCTTCTAAACGATGGCATTCTATGTAATGCCTTTCCTG
  GACTTTTTTGGCCACTGCCCTGGACTAGTGAAAGAATGGACTCTATCTTTATCTGCAAGAGGAACTAAG
  GCCTTCTCTCAGACTGCCTGGCCAGCCTGGGGCACTGAAAATACGGCTCATGTTAATGAGTTACATTAT
  CAGCCAGCCCAGCCTTGCCCACCATTTAAGAAATATCACAGAGCCACTAGATCTCATATGATCTTCTTC
  AAGCCATTATTTTAACTCAAGAAAACTCTAGAGAAGAAAAGTGAAGAAGTCATGTTGAAGAAGATGTAA
  GAATGTGTCAAGACCATCCAGAAATGATATGAGAAATACTGATATTTTAAATGGTTGACATCATCCAGC
  GAAATGAATCTACATTAAATGTTGTTTTAACTGCGCTATGATTAAAACCATTCATATAGAGTTAGTCTT
  TACAACTACTATTCTGTTATTTTTTTTTTTAATCTGACAACATTTGTCCTAAGTAAGATAAGCAAAAAA
  ATTCTTCAACTCCTTTTGGCAAGAAAACTGTAACAGAAAATAAATTTTGAATGTGTACTTAAGTCTTTA
  TTATATTTGAAGCAATTTTTTTTCAATTTTAAAAGCTGAATGAAGACAACTTAGGTTGCTAACCTAGTT
  CAAAATGAAATTATTTAGATACCAATTTTTAAAATACTGGAGAGAATTTATATGTCTTTTTCCAGAGTT
  CTGATGATAAGCATTTGGAGTGCATTTATTCCTCCAGATAATAAATGTGTGTTCAGAACTTTTTGTGTT
  TTTTAAGGCATTAATAAAGCCTTCGATAATATTAAATACAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_004572.4
• Summary: This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene product may regulate the signaling activity of beta-catenin. Two alternately spliced transcripts encoding two protein isoforms have been identified. A processed pseudogene with high similarity to this locus has been mapped to chromosome 12p13. [provided by RefSeq, Jul 2008]
• Locus ID: 5318
• MW: 66.6