Kir6.2 (KCNJ11) (NM_001166290) Human 3' UTR Clone

CAT#: SC215939

3' UTR clone of potassium inwardly-rectifying channel subfamily J member 11 (KCNJ11) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: KCNJ11
• Synonyms: BIR; HHF2; IKATP; KIR6.2; MODY13; PHHI; PNDM2; TNDM3
• ACCN: NM_001166290
• Insert Size: 1701 bp
• Sequence Data:
  Insert Sequence

  >SC215939 3’UTR clone of NM_001166290
  The sequence shown below is from the reference sequence of NM_001166290. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TTCAGCATCTCTCCAGATTCCCTGTCCTGAGCCATGGTCTCTCGGGCCCCCCACACGCGTGTGTACACA
  CGGACCATGTGGTATGTAGCCCGGCCAGGGCCTGGTGTGAGGCTGGGCCAGCCTCAGCTCAGCCTCCCC
  CTGCTGCTCATCCAGGGTGTTACAAGGCACTTGTCACTATGCTATTTCTGGCCTCAGCAGGAACCTGTA
  CTGGGTTATTTTTGTCCCTGCTCCTCCCAACCCAATTCAGGACTGGCTCACCCCTCTCCCCCGCCCAAG
  GCTGCAGAGGCTGTGGGAGGTACTGGGCCCTAGAGCTGTGCGTCCAGCCAGTCCTGGGTCCCCACGATT
  GACCAGCCACACTCTGGGCCGGTGGCTGGGGAAGAACAATCCCCGAGGGCTGCTGCTTTGCGTCTGTGG
  CTCCAAGAAGTGCCTGTGGTCAGGCCCCAGCTCTACTTGGTCCCTGAAAAAGCACCTGGCTAAGGGCTG
  GGCCTGGCCAGCAGGGAGGGCAGTTGATGAGAGAGGGTGTTCCCGCTGGAGGGTTGGTGCTGTGGAGCC
  TACACCGGCAGGGACAGCCTGGGGCTGACAGGGCTCCCCTCCGAGGGCCAGTTTCAGGTCTGGAAGGGG
  AGGAAGCAGGGGAAGGTGACCTGAGGAGGCTCGGCTTTGTAGAGCCCCGCTCAGGCACAGGGAGGAGGA
  GATGCCAGGGCTCCTGCCTTTTGCCACATCGGCCTCGTGCAGTGAGGGCTCTGTGGGCTGGGGCTGCTG
  CCCCTGCCTACCTCCTGCCTGTCCCCAGAGGCTGAGGAGAGGGGGTACTGTGCCCACCACACATGATTA
  GGCCTCAGACCCAACTCTGGTCCTGGCTCCACAACAGTGGCTGCCACTCACTTTGTCCAGAAGGTGGCT
  TGGGGGTGGATATCTTTGGGTTGCTGGAAAAGGTGTGGGAAGGTTCAGGATGGTGGGAGGGACTGAGGT
  CCCTGAGGTGAAGAGGCCCTTGGTCCTGACGGGTTTGACCCGTGCCTGGACCCTTGGAGCAGTGTTGTG
  TGAACTTGCCTAGAACTCTGCCTTCTCCGTTGTCAATAAAGCCTCCCCCTCATGACCTAAACTCTGGGC
  TTTTCTTGCTGGGGAGGCAGCAAGCATGCTGGTGGGAAGGGAGGCAGGGACTGGCAGCTGCCACCCCCT
  TCAAGAGGCGCCATAGACCCTAGCGGGGAGGGCAGGGGAGGGACGGAAGGCTGGCACCTCTTCCACCAG
  TTCAGGGGGACTTTCCCCTCTCCTGTCTCAGGTGGCCCAGCCCTGTCAGCCTGTCTGGCCAACTCAGCC
  TTTGGGCACTCACCAGGCTTTGCAGCCCTGGGCTCTGTCTCTACTCCCAGGGACCTGCTGGAAGGCTGG
  AGTGCCCAGGGAGAGGTATAGAGGTGTCATAGGCATTAGTGTAGTAATTGGAGCACTAACTCTCGAGCC
  AACTGCCTGGGTTCGAATCCTGGCTCTAGCTGTATGACTTTTGTCAAGTAACTTAGCCTCTCTGTGTCT
  CAGTTGCCTCTTCTATAACATGGATGCTAATAGTACCTACCTCATAGAATTGTTTTGGAAGTAAATGAA
  AAATATGTAAAATGCTGAAGTGCCTGGTCTACAGTAAGTGCTCAATAAATGTTAACTATTGTGATTGCT
  GCTGAATCAGCTACATGCTGAGGAAACGGCCAAACAAGTGTTAAA
  AGCGGACCGACTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCC
  CAACCTGCCATCACGAGATTTCGATTCCACCGCCGC
  
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001166290.2
• Summary: Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and is found associated with the sulfonylurea receptor SUR. Mutations in this gene are a cause of familial persistent hyperinsulinemic hypoglycemia of infancy (PHHI), an autosomal recessive disorder characterized by unregulated insulin secretion. Defects in this gene may also contribute to autosomal dominant non-insulin-dependent diabetes mellitus type II (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM). Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2009]
• Locus ID: 3767
• MW: 60.1