TPM4 (NM_003290) Human 3' UTR Clone

CAT#: SC215853

3' UTR clone of tropomyosin 4 (TPM4) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TPM4
• Synonyms: HEL-S-108
• ACCN: NM_003290
• Insert Size: 1686 bp
• Sequence Data:
  Insert Sequence

  >SC215853 3’UTR clone of NM_003290
  The sequence shown below is from the reference sequence of NM_003290. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CAGACACTAAACGAACTTAACTGTATATAAGCAAAACAGAAGAGTCTTGTTCCAACAGAAACTCTGGAG
  CTCCGTGGGTCTTTCTCTTCTCTTGTAAGAAGTTCCTTTTGTTATTGCCATCTTCGCTTTGCTGGAAAT
  GTCAAGCAAATTATGAATACATGACCAAATATTTTGTATCGGAGAAGCTTTGAGCACCAGTTAAATCTC
  ATTCCTTCCCTTTTTTTTTCAAATGGCACCAGCTTTTTCAGCTCTCTTATTTTTTCCTTAAGTAGCATT
  TATTCCTAAGGTAGGCAGGGTATTTCCTAGTAAGCATACTTTCTTAAGACGGAGGCCATTTGGTTCCTG
  GGAGAATAGGCAGCCCCACACTTTGAAGAATACAGACCCCAGTATCTAGTCGTGGATATAATTAAAACG
  CTGAAGACCATAACCTTTTGGGTCAACTGTTGGTCAAACTATAGGAGAGACCAGGGACCATCACATGGG
  TAGGGATTTTCCATCCAGAGCCAATAAAAGGACTGGTGGGGGCCGGGGGTGGCTATTGTGGGAAGTCAT
  AACCCACAGATAGATCAACCTAAGAATCCTGGCCCTTCTCCACTCTCCACCATGCAGGACAAACATCTT
  CTCAAGCAGTCAACGTAGAATGCTTGGGAAATAGTCATAATTACCCACATATAGTAATTAATAGATGGT
  AATTAATTGATCCTTGATGTGATGTTCTTTTGCATATTTCCTTCATTCTAAAGTTGTTCCCTGGCCGGG
  AGCGTTGGCTTTCGCCTGTAATCCCAACACTTTGGGAGGCCAGGACAGATCACTTGAGGTCAGGAGTTC
  GAGACCAGCCCAGCCAACATGGCGAAACCATGTCTCTACTAAAAATACAAAAATTATGGTGACGCCTGC
  CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGGATCGCTTGAACCCAGGAAGTGGAGACTGCAGT
  GAGCCGATATCGCACCACAGCGCTCCAGCCTGGTCGACAGAGTGAGACTCCATCTCAAGAAAAAATAAA
  AATAAAGTTGTTCTCTGAAGAGCAAATGTCTCATTCCAGTAATGACCCACTCAGCAGGAATATGGTGGA
  GTTCAGTCCAATTCAGGTCAGCCATATCCAAAAGACCACAAGTCATTACTAAGTTGAGCAAAAGAGTTT
  TTATCTATTAGCAGAAAGGGCCTCTCTGGCAGCAGAGATTAAAAACTGGCCCAACTTCATTTCCATACT
  TCAGGGAACAGCAAATTGAGGATTTACTTATCTAGGACTTGAATTCCTTCTTTGGGACCAAGTTAATAA
  AAGACCAAGAAACTCCTGATTAAACTGGATAATGAAGGATTCTGTAGACAGGGCTGCACGTATCGGCTT
  TGTTTGACTTCTCTTTTCTCAGTTAACATCTCAGAGCTAGAACATTCCACATTCCCCAGCAGCGTGTGG
  GGGCTGACTAAAGTTTACAATTCCAACTAAAAATCACCCTGCTTCTGGCTTATCTGAATCCCTTACCCA
  CCCCACCCCACCACCCTACTCCTATTTATTCAGCACCACACTACCCAGGAAATACACTAGCAAATTGTG
  CAATGGAATAAAATCCACACTTTAGATTCTTGCAACTGTATCATATGTAATAGTATCACTTTTTCTACA
  TTTTGGTCAAATAAATTTTTACATAAACTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003290.3
• Summary: This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009]
• Locus ID: 7171
• MW: 63.7