AKR1D1 (NM_005989) Human 3' UTR Clone

CAT#: SC215772

3' UTR clone of aldo-keto reductase family 1 member D1 (delta 4-3-ketosteroid-5-beta-reductase) (AKR1D1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AKR1D1
• Synonyms: 3o5bred; CBAS2; SRD5B1
• ACCN: NM_005989
• Insert Size: 1673 bp
• Sequence Data:
  Insert Sequence

  >SC215772 3’UTR clone of NM_005989
  The sequence shown below is from the reference sequence of NM_005989. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCTGAATACCCATTTCATGATGAATACTGACTGCAGGGAGTTCCTGAACAGATTTTTCACTCCCACGAG
  TGCCAAGACGGTGCAATGGGTAGTCCCCTAGATGTGAAAATGAAGAGAGAGGGTTTTACCATCCTGAGA
  AGAAATAATGATGGAAACATGTTTAATGTTTGTGCAGTGTAAATGACTTTGACTCAGTCACATTGAAGT
  AAAAATATTAAAATCTGTTGAAATAACTCTTAGGAAATTATCAACTAATTTTTTCAGATCAGTATCTTC
  TAGATTCCAGACAGAAAAAAATTACACTTCAGAAAAGACATCAAAGGCAACATATGACAACAAGTAATT
  TATGAATCTGGGTAGTAGCGTTGGTAATCTGAGTTCTTTAAGGGTTAACAGGACAACGAAGTGCATGTG
  GCAGTGTGCTGGCAGTGGCCTTGAGGCTTTGGACCATTGGTTACAAAACAGACACAGCCAAGATAAGAT
  CCACACACACATTATTAACAAGGAAGTGATTTGCTGCACCTTGAGTTGAGAGGGCTACATGTAGAAAAG
  TCTTAAAATAGAGCTAAACACCACAGTGGTCAACAAAGCCATCATAATGTTGGTGTTTGTTTCCCTCCA
  ATGTATGTATGTTTAGTTTTTATCCAACCTGAGGAATGAAAACTTAACTGGATCTCTCTTGCATCCTTA
  AAGGGCCTGAGTCTCAACATGGCTGCTGATCCATACTTACACATCTTACTGTCAATCTTGCCTACATTG
  ATTATAGAACCACTATTACGTGAAAAGGCTTGAAACAACCAACATATACAAATAAAACCCTGCCTTGTA
  AAATAGTAAAAGAGAAGCCATATATTGGCTTTTCTTCTTAACTTGGGAGATATATTGAAACAAGGTGCT
  TTATAAGATTATTGTACTTAAGACTTTAATAGTGTTACTTGGATAGCTTATATGAATTTTGAGAATTTT
  ATATGAATTTTGAGAAAGCAAGTTCAAAAGAACTCTGGTAATTTTCCTGTATGTACAATTTAAAGAGTG
  AATAAGATTATTAGAATTCAGCAATAGAGATATATCTATTTTCAATTCAACTACAGAAATATATTTTAT
  TGGCCGGGTGCGGTGGCTCATGCCTATAATCCCAGCACTTTGGGAGGCCAAGGTGGGCAGATCAGGAGG
  TCAGGAGATCGAGACCATCTTGGCTAACAAGGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGC
  CAGGCGCGGTGGCGGGGGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGAAGAATGGCATGAACCC
  GGGAGGAGGAGCTTGCAGTGAGCCGAGATAGCGCCACTGCAGTCCGGCCTGGGTGAAAGAGCGAGACTC
  CGTCTCAAAAACAAAAAAAAAAGAAAAGAAATATATTTTATTCATTCACATTAGGTCACTGTCATACTG
  TCATAGGATGAGAGAGTTCTTCAAAAATTATGTTTTCCCAAGATCAGTTGCTTATAGATAATGTTCAAT
  GACCTCAAGACATATATTTTTGAGAAATTATCATTTTAAAAAATTTGGTCTATACTGATTGTTTTCACT
  GATTCCAATATTATTACTTATAACACTGACCTCTGGAAAATATTTTGTTCACAAGAAATAATAAAGTAT
  AATGATTTGTTGCATCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005989.4
• Summary: The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. [provided by RefSeq, Jul 2010]
• Locus ID: 6718
• MW: 64.7