FRAT1 (NM_005479) Human 3' UTR Clone

CAT#: SC215681

3' UTR clone of frequently rearranged in advanced T-cell lymphomas (FRAT1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: FRAT1
• ACCN: NM_005479
• Insert Size: 1652 bp
• Sequence Data:
  Insert Sequence

  >SC215681 3’UTR clone of NM_005479
  The sequence shown below is from the reference sequence of NM_005479. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGCGACGGCGTTCTTGTGCCTGGCAGCTAACACGCCCGGGGTGGCCACAGCGCCAGCCTCAGACTGGAG
  GGCAAGGGGTTCCCTTGAGGGCTGCAGTTCTACTCAGGCTGGTGGAGAACTCTGGCTTTTGGAAGCGAG
  AGTAAAAAGCTAATGACGAGGAACCGAAAAATCGCGAGTGTTTCGCGGGTAACTGGGGTTGAGGGCCAA
  AATATTTGGAATGAAGGACTTTGGCCCTATTTAAGGCAGATTTTACAGAGCGCACCTCAAACGTACAAG
  TCAGTAGGACTCCTTATTTGGCGTGACCCGACCTGGCCGCGGAGCCTGCATTTCCTCGCAGCCTCTCAG
  TGCCCTCCAGCCCCGCGACCATGTGGCCACAATCCACGCTTCTCCGGATCGCGGTGCGCCGGAACCACG
  GAGGATGATGCCAGTTACTTGCTTTACCTTTTCAGGGCTGGCTCCTGATCCACTTTGGGGGAGGAGAAC
  ATGAGTAGATAATTTCAGGGTGCAGCCCAATCTGCCAGACTTAAAAAAACCATCTTGTGTCTTTGGAGG
  TGCTGCTTAATACCAAACATGCGGTGCCATGAAGGGACCCTTTGGGGGTTGAATAGGAGTTAACCCCTG
  CGCTCTCTTTGCAACTGTCTCTCTTCTCAGAGTGGTGGGGGAAGGCTGTACGACACGGGTGGGGAAAGG
  AGGTGGGGGCGGGGAGTATTGAATGGTGGTGGAAGGGTAGAGAGGCGCGGAGTGAACCCCACGCCCTGT
  CTAAAGTGTATTTTCAGAGCCGGCCCGCCTCTCCTCGGTTCAAGGTCACTGTTTCCTGGGCACGCACTG
  GGTTGCGGGACAGAGTAGCCAGGTTCTGCCGGTGCTCGGAGAAGAGCGCAGTGTTTTGCAAGTGCTGGA
  GTCTCCTGAGGACACGCGCGTCGCCGCCACCGCGGGTGTGGGAAAGCGCGGACGTGCTGGGCGGCTGTG
  CTTCGGTAGGCGACCACCGCCCCTGGCCGCGCTCCGGGCTTTCACGGAAACTCCCGAGACCGGGCCCTG
  GGTTCCTCCTCTCCTACTCGGCTCTGCAGTCCTACTCAAGCGGGTGGCTCTGGGATCCTGGGGGCCTGG
  GTTGGGGGCTAGGGAGACGCCATGTGATGGACACTCCAGGGACACACAGCCTAGCACAGCAGCTTATAA
  TGGGCTCTCCGGGGCCATTTGCAATAACAGCTGCAATTCCCTGGATAGACGAGTTGATTTCCTCCCTCT
  GCCCCTCCCCCAGCCATGCCAGCTGGCCTTTGTAAGTGCAGGAAACCGAGTAGAAAATGTGACCCTCCA
  AATGGAGAAGCTGCAGGCTTTGCCATTGTGAACCATGGTGAAGTGCTTGGAACATACTGTTCACTCACT
  CTAAAGGCGCTGAGACTGTGCTGTTGTTCTCGTTTTTATAGTCAATGGCTTGTTCATCATCCAGATGTG
  GCTACTGACATATCTACACTTCGCACCGGAGTGTCTGGAATTGTGGCTATCCTGATTATAGGATTTTAA
  CTTAACTGAAATGCCTGCTTTGAATAAATGTGTTGGGTTTTTTGTTTGGTTTTATTTTATACTTGCCAT
  CAGTGAAAAAGATGTACAGAACACATTTCTCTGATCTCCATAAACATGAAAACACTTGAAATCTC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005479.4
• Summary: The protein encoded by this gene belongs to the GSK-3-binding protein family. The protein inhibits GSK-3-mediated phosphorylation of beta-catenin and positively regulates the Wnt signaling pathway. It may function in tumor progression and in lymphomagenesis. [provided by RefSeq, Oct 2008]
• Locus ID: 10023
• MW: 59.7