ZFYVE27 (NM_144588) Human 3' UTR Clone

CAT#: SC215644

3' UTR clone of zinc finger FYVE domain containing 27 (ZFYVE27) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ZFYVE27
• Synonyms: PROTRUDIN; SPG33
• ACCN: NM_144588
• Insert Size: 1625 bp
• Sequence Data:
  Insert Sequence

  >SC215644 3’UTR clone of NM_144588
  The sequence shown below is from the reference sequence of NM_144588. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GCCTCGTGTAACCAGACCTTGAGCAAGTGAGAAGAGAGGCCAGGGTCCAACCAGGCACCCGTCCTTGGG
  ACCAGCAGTAGACCCCCCACTCTCCCCACCCCTGGCCCACTGTGGTGTGTGCTGGGCAAATGTGGCCTG
  AATGCTAGGTAGGCTTCCCCTTCCTTCCTCACTCTCTCCAGCTGGATTCTGGAGCTGTTCTCCATCCAT
  GAGAGTGGCTGGCAATGGCTGCTCTCAATCCCTTGAGGGAGAAGAGCCCCTGGAGGGCCTGGCATGTTT
  GTCCTGCTCTGCCTGGGACTGAGCGAGTGGACTTAGGGCTGGGCAGGCAGTAGCCACCAGAGGGCAGCA
  GCGAACTAGGCCAGGCCTGACTGGGGTCTGAAGATCAGGGTCAGTGTGGCTGTGCCTGGGAATTCCAGA
  CCTGAGGTTGGGAAAAGAGGTTTTTCTCCTGCAGGGTACTGGGCCAGGCCCTCAGCCTCAGAGAGCCTG
  CAGAAGGGCTTGGGAGTGCCACACCCCATCTCTGCTGATTGAATGTCCCTCCAGGCACCAGGATCTCAT
  CATTTCCCCATCAGAGGGTGTGGCCAGGCCTAACAAGACCATGGGTGCTTCTAGAAACAGGGTTGAAGT
  TCCCAGATTCCCTGAGAGGAGAATGTGTATAGGAGGGTTTGGCTGAGTCCTTCAGCGTTAAGTGGAGGA
  AAGCTTGGGGAAGCCCCAATAGCTGGACAGACCTCGGCCTCCCCTCGAAGACACCTCAATTCACAGACT
  CTCAGCCCACACAATGCCCCAGTGTCCCCAGCTCCGCTGGAGCAGCTGCAGGGCACTTGGATCACAACT
  TCTGCACCCTCTGTCCAGAGTCTAGGGCAGTCCTCCACTGGCCCAGCACTCCAGTTTCCTTTCCCTGCC
  TCTTGTCCAATGGAGTGGGAGGCCAGGTGAGTGGAGCAGAGGTCCTGAAGCCCTTGACCCCTGGGGGCC
  TGGGTAGTGTAGGATCTCGCTGGGCTGGGTCCTGGATTCCAGGGCTATTCCCTGGAGGACAGTCTCAGT
  TATGGGATAAGGCCCCCTGGGGGTCTCCATTTCTTTCCAACAGTTTCATGTTCACTACTGGACTCTTAC
  GGGCTCAGTATCTCTCCCTTAGCCATGAGCTGGCTCAGGCATCCCTTCCCTTCCCTGGAGCTGCCCTGC
  CTTTCTCAAGTATTTATTTATTTATTGCATGGTTCCTGGGAACATGTGGCACAAGTAATGGGATGAGGA
  GGAATTGGGGGTGGGGGTCTTCTACCTAGGACTCTTCCCTGGAGTCATGGGCTGCCTGGGACCCAGGAC
  CCATGAGGGGGCTGAGAGGTTTCTACACTCGAGGAGCAGGGGTCCAGAGAGGCAGGCTGGGGAGGCAAG
  GGACCCATCCTAGGCCCGCTTTCTTGCCGAGCCAAGCAGCTTAGCTGGGGCTGTGCAGCCAGGGGCTTA
  CCCAGGCCAGTGGAGGTGCCACAGCCCTGGGGAGCCAGACAGGCTTTGGTATCGTATCGCCTCTGTGTC
  CTTTTAAGAGAGGAGAGTTCAGTACCCCGTGCTTTCTTTACACTGGAGAGGAACTAAAAGGATCTCTGT
  GTCTATGGAGAATTGTCAATAAAAAGGCCTCAAGCTTC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_144588.7
• Summary: This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear. [provided by RefSeq, Mar 2010]
• Locus ID: 118813
• MW: 57.9