Myelin Basic Protein (MBP) (NM_001025092) Human 3' UTR Clone

CAT#: SC215636

3' UTR clone of myelin basic protein (MBP) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MBP
• ACCN: NM_001025092
• Insert Size: 1624 bp
• Sequence Data:
  Insert Sequence

  >SC215636 3’UTR clone of NM_001025092
  The sequence shown below is from the reference sequence of NM_001025092. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CGCTCTGGATCACCCATGGCTAGACGCTGAAAACCCACCTGGTTCCGGAATCCTGTCCTCAGCTTCTTA
  ATATAACTGCCTTAAAACTTTAATCCCACTTGCCCCTGTTACCTAATTAGAGCAGATGACCCCTCCCCT
  AATGCCTGCGGAGTTGTGCACGTAGTAGGGTCAGGCCACGGCAGCCTACCGGCAATTTCCGGCCAACAG
  TTAAATGAGAACATGAAAACAGAAAACGGTTAAAACTGTCCCTTTCTGTGTGAAGATCACGTTCCTTCC
  CCCGCAATGTGCCCCCAGACGCACGTGGGTCTTCAGGGGGCCAGGTGCACAGACGTCCCTCCACGTTCA
  CCCCTCCACCCTTGGACTTTCTTTTCGCCGTGGCTGCGGCACCCTTGCGCTTTTGCTGGTCACTGCCAT
  GGAGGCACACAGCTGCAGAGACAGAGAGGACGTGGGCGGCAGAGAGGACTGTTGACATCCAAGCTTCCT
  TTGTTTTTTTTTCCTGTCCTTCTCTCACCTCCTAAAGTAGACTTCATTTTTCCTAACAGGATTAGACAG
  TCAAGGAGTGGCTTACTACATGTGGGAGCTTTTGGTATGTGACATGCGGGCTGGGCAGCTGTTAGAGTC
  CAACGTGGGGCAGCACAGAGAGGGGGCCACCTCCCCAGGCCGTGGCTGCCCACACACCCCAATTAGCTG
  AATTCGCGTGTGGCAGAGGGAGGAAAAGGAGGCAAACGTGGGCTGGGCAATGGCCTCACATAGGAAACA
  GGGTCTTCCTGGAGATTTGGTGATGGAGATGTCAAGCAGGTGGCCTCTGGACGTCACCGTTGCCCTGCA
  TGGTGGCCCCAGAGCAGCCTCTATGAACAACCTCGTTTCCAAACCACAGCCCACAGCCGGAGAGTCCAG
  GAAGACTTGCGCACTCAGAGCAGAAGGGTAGGAGTCCTCTAGACAGCCTCGCAGCCGCGCCAGTCGCCC
  ATAGACACTGGCTGTGACCGGGCGTGCTGGCAGCGGCAGTGCACAGTGGCCAGCACTAACCCTCCCTGA
  GAAGATAACCGGCTCATTCACTTCCTCCCAGAAGACGCGTGGTAGCGAGTAGGCACAGGCGTGCACCTG
  CTCCCGAATTACTCACCGAGACACACGGGCTGAGCAGACGGCCCCGTGGATGGAGACAAAGAGCTCTTC
  TGACCATATCCTTCTTAACACCCGCTGGCATCTCCTTTCGCGCCTCCCTCCCTAACCTACTGACCCACC
  TTTTGATTTTAGCGCACCTGTGATTGATAGGCCTTCCAAAGAGTCCCACGCTGGCATCACCCTCCCCGA
  GGACGGAGATGAGGAGTAGTCAGCGTGATGCCAAAACGCGTCTTCTTAATCCAATTCTAATTCTGAATG
  TTTCGTGTGGGCTTAATACCATGTCTATTAATATATAGCCTCGATGATGAGAGAGTTACAAAGAACAAA
  ACTCCAGACACAAACCTCCAAATTTTTCAGCAGAAGCACTCTGCGTCGCTGAGCTGAGGTCGGCTCTGC
  GATCCATACGTGGCCGCACCCACACAGCACGTGCTGTGACGATGGCTGAACGGAAAGTGTACACTGTTC
  CTGAATATTGAAATAAAACAATAAACTTTTAATGGTA
  AGCGGACCGACTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCC
  CAACCTGCCATCACGAGATTTCGATTCCACCGCCGC
  
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001025092.2
• Summary: The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]
• Locus ID: 4155
• MW: 60.6