RAD50 (NM_005732) Human 3' UTR Clone

CAT#: SC215339

3' UTR clone of RAD50 homolog (S. cerevisiae) (RAD50) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RAD50
• Synonyms: hRad50; NBSLD; RAD502
• ACCN: NM_005732
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC215339 3’UTR clone of NM_005732
  The sequence shown below is from the reference sequence of NM_005732. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GTTAGCTCCCTGGGATTCAATGTTCATTAAAAATATCCAAGATTTAAATGCCATAGAAATGTAGGTCCT
  CAGAAAGTGTATAATAAGAAACTTATTTCTCATATCAACTTAGTCAATAAGAAAATATATTCTTTCAAA
  GGAACATTGTGTCTAGGATTTTGGATGTTGAGAGGTTCTAAAATCATGAAACTTGTTTCACTGAAAATT
  GGACAGATTGCCTGTTTCTGATTTGCTGCTCTTCATCCCATTCCAGGCAGCCTCTGTCAGGCCTTCAGG
  GTTCAGCAGTACAGCCGAGACTCGACTCTGTGCCTCCCTCCCCAGTGCAAATGCATGCTTCTTCTCAAA
  GCACTGTTGAGAAGGAGATAATTACTGCCTTGAAAATTTATGGTTTTGGTATTTTTTTAAATCATAGTT
  AAATGTTACCTCTGAATTTACTTCCTTGCATGTGGTTTGAAAAACTGAGTATTAATATCTGAGGATGAC
  CAGAAATGGTGAGATGTATGTTTGGCTCTGCTTTTAACTTTATAAATCCAGTGACCTCTCTCTCTGGGA
  CTTGGTTTCCCCAACTAAAATTTGAAGTAGTTGAATGGGGTCTCAAAGTTTGACAGGAACCTTAAGTAA
  TCATCTAAGTCAGTACCCACCACCTTCTTCTCCTACATATCCCTTCCAGATGGTCATCCAGACTCAGAG
  CTCTCTCTACAGAGAGGAAATTCTCCACTGTGCACACCCACCTTTGGAAAGCTCTGACCACTTGAGGCC
  TGATCTGCCCATCGTGAAGAAGCCTGTAACACTCCTCTGCGTCTATCCTGTGTAGCATACTGGCTTCAC
  CATCAATCCTGATTCCTCTCTAAGTGGGCATTGCCATGTGGAAGGCAAGCCAGGCTCACTCACAGAGTC
  AAGGCCTGCTCCCTGTAGGGTCCAACCAGACCTGGAAGAACAGGCCTCTCCATTTGCTCTTCAGATGCC
  ACTTCTAAGAAAAGCCTAATCACAGTTTTTCCTGGAATTGCCAGCTGACATCTTGAATCCTTCCATTCC
  ACACAGAATGCAACCAAGTCACACGCTTTTGAATTATGCTTTGTAGAGTTTTGTCATTCAGAGTCAGCC
  AGGACCATACCGGGTCTTGATTCAGTCACATGGCATGGTTTTGTGCCATCTGTAGCTATAATGAGCATG
  TTTGCCTAGACAGCTTTTCTCAACTGGGTCCAGAAGAGAATTAAGCCCTAAGGTCCTAAGGCATCTATC
  TGTGCTAGGTTAAATGGTTGGCCCCCAAAGATAGACAGGTCCTGATTTCTAGAACCCGTGACTGTTACT
  TTATACAGCAAAGGAAACTTTGCAGATGTGATTAAAGCTAAGGACCTTAAGACAGAGTATCCTGGGGGT
  GGTGGTGGGGTGGGGGGGGGTCCTAAATGTAATCACGAGTAAGATTAAGAGCAAATCAATTCTAGTCAT
  ATATTAAACATCCACAATAACCAAGATATTTTTATCCCAAGAATGCAAGATTTCAGAAAATGAAAAATC
  TGTTGATAAATCCATCACTATAATAAAACCGAAGGTGAAAAAAATTCTGAAAAAATTCTAGCAGCTATA
  TTTGATAAAATTCAACATCTCCTAGCTTTAGCAAACTCACAGTTTTGCAAATAATATTTTCTTAATGTT
  ATCTGTTGCTAAATCAAAATTAAACAGTCATCTTAACTGCAAAATAAAACATTTCTCAGTAAATATTAA
  AGCCAGTTACCTTCTATCAACATGTTAATGAAAGTGCTAGTTGTTGCAGCAAAGAATAACAAAGGCAAT
  ACACGATCAATATAGGCAGTGAAACAAAAGTATCATTTGCAAGTTAAAACAGACTTCCCAATTTTAAAT
  CTGGTTTCCCCCTGAATATGTGGCATCCTTGGCAGCACTTCTGAGAGTGGCTGCTTTCATTCCAAGAAG
  CCCATGGGTTTGGAGGTGGGATAGGTGCCTTTCTGGCTTCTCATTGCTGCTTCTAGATCAGTCTCCAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005732.4
• Summary: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]
• Locus ID: 10111
• MW: 74.5