B3GALT2 (NM_003783) Human 3' UTR Clone

CAT#: SC215324

3' UTR clone of UDP-Gal:betaGlcNAc beta 13-galactosyltransferase polypeptide 2 (B3GALT2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: B3GALT2
• Synonyms: beta3Gal-T2; BETA3GALT2; GLCT2
• ACCN: NM_003783
• Insert Size: 1594 bp
• Sequence Data:
  Insert Sequence

  >SC215324 3’UTR clone of NM_003783
  The sequence shown below is from the reference sequence of NM_003783. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGCAGGTATCGCCACCGTAAACTACATTAGAAAAGACAATTTTTTTTCAAATGTGCAATTTGTAAATAT
  TGCTAAAAGCATGTATAGTTAGAACTGATTACATCCGTAGGACAAGTTTTAGTTAAAACTCATCACATA
  AAGAAATTCAAGAAGTATTTTTTTAATTTCTGAAGAAGTTAATTCTTAAAACTATAACATTATATAACA
  AAAAAGGTTTCCCAAAACAATCTATTTAAAAAACTGTATAAGGAGATTCTGTGTATTAACATGCAATAA
  CAAGCATGCATAAATCAATGGTTCAAGTCTTCTGTTAGGGGCCAATAAAATGTATCTGCATATGTTTTC
  CACATAAATTTTAATTCAAGAAATGACAGTCAAAAGATCCTTCATTTTAGATTAAGCTTTTCATTTTAA
  TATATAATTTAATGTAAATAAAACATCACTATCAATTTTAAGGAAACTTTTTAATTGTGCAAAGGATAA
  ATTTTTTGACCTATTTTAGGGTTCTAAATGCAATAAGATTTAGTTGAGTTATTCCACAAACACATTATA
  AAGTTCAGATGTTTCATCAATGCAGTTCTCACGAAAGTATTTACTTTTTAAAAATAACTGAGATATTAT
  TTTAAATTTCTTTTATTAATACTTTCTTTTATTAATATATGGGGGAAAATTATTTTGACATGACGTGGT
  AAAATGTGAAAAACTAATGTGTCTCAGGCTCAAGTTTTTATAGTTATTAAATGTTTCAAAATAGACAAG
  TTTTGTTTCCTCATTGATGTTAAGAACCAAACTCCTATTTCAATGAGTTATTGGATTAGACCAATTACT
  GCACTCTTAAACAGCACCACCATTTAATTTCATGTATATCTAACTTCGAATATATCTGTAAAGATAATC
  GAAGCAAAAGTAATCACTTAAAGGCACAAATAGGATGTACTGTTGAAAAAGATAAAGAGTGCAGGTGCA
  GTTTCATTCAACACATTTTTAAGATGCATGTCTGCCAAAATGCAACATACGGGAAGTTTATTTCCTGAC
  AGCAGGTGTACACATGCCAACACTTAATCATTTTATGGCACCTATTTCTTTCTTGGAGTGCCAAGTTTG
  CAAACCTGCAGTTTTTAATTTGGTAGATGACAAATATTCTGAATCACCAATTAAAAACCTTTTTGGGAG
  GGATGGGGAAAACTACAAACGTTTGACAAACACAATTCTAGGATGAACAATGTATACAATGCACTTTTA
  TGAAGTTTTTAAAAATAAAGGAAAACAAAAAACTTTACTGAGTGTTATAAGTATTTTCACAATAATTTT
  GTTTGACTCCATCATGAATTTTAAAATCAAGAAATACCAACCTACTAACTAAAATAGTGTTTTTATTCC
  ATACAAGTATGTAAATCTGTCAATCTTAATTTAGTCAGAATGTTTCTTAATTCTAATAACAATCATTTT
  TACCTCTTATTCTATTATTAAGGGATAAATCACCAGAAGAGAATTGTAACATTTGTGAAACTTGAAAAC
  ACTAAAACTGTTTAAGTGCTTATCTCCATTTCAAATCAGTAGCCTAAGTGTTCTACTCTAAATCAAGTG
  ATTCTGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003783.3
• Summary: This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene encodes a protein that functions in N-linked glycoprotein glycosylation and shows strict donor substrate specificity for UDP-galactose. [provided by RefSeq, Jul 2008]
• Locus ID: 8707
• MW: 63