CYP51A1 (NM_000786) Human 3' UTR Clone

CAT#: SC215039

3' UTR clone of cytochrome P450 family 51 subfamily A polypeptide 1 (CYP51A1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CYP51A1
• Synonyms: CP51; CYP51; CYPL1; LDM; P450-14DM; P450L1
• ACCN: NM_000786
• Insert Size: 1542 bp
• Sequence Data:
  Insert Sequence

  >SC215039 3’UTR clone of NM_000786
  The sequence shown below is from the reference sequence of NM_000786. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GTTATCCGTTACAAACGAAGATCAAAATGAAAAAGGTTGCAAGGAACGAATATATGTGATTATCACTGT
  AAGCCACAAAGGCATTCGAAGAGAATGAAGTGTACAAAACAACTCTTGTAGTTTACTGTTTTTTTAAGT
  GTGTAATTCTAAAAGCCAGTTTATGATTTAGGATTTTGTTAACTGAATGGTTCTATCAAATATAATAGC
  ATTTGAAACATTTTCTAATAGTTATGATACTTATACATGTGCTTTCAGGAAGTTCCTTGGTGAAACAAT
  TGTTGAGGGGGGATCTAGGTAATTGGCAGATTCTAAATAATATAATTTCCAGATAGTAATTTTAAGAGT
  ACTCATCGCTCTTGCCAAATAAGTTCAGGGTATTCAAATCTTGGACTAGTCCTGCAAGGTATAAAGAAT
  AAAAATCCCAGTGAGATACTTGGAAACCACAGTTTATTATTATTTATCTGGGCAATTATTGTGTGTGTG
  AGGATGGAAGGGTAGGGAATAATCGAACATCTAAAGCCTTGAATAAGAGAATACTAATTGTTTTGGTAT
  GATGATACTCAGAAATGGAGATATTATAGGAAAAAGAAATCCTTTGGAATTTTAACTAAAATCACTGCA
  TATGGGAAATTAAGAGATCCAGGACCATATTTGATAAGAGTTCCTAAAAATAATGTAATTATTAATGCT
  AAAGACTGCTCATGTATCTTGATCTAATTACTAAATAATTACATATTTATTTACCTGATAAATATGTAT
  CTAGTTCTACAAGGTCACATTTATGTGGAAGTCCAAAGTCAAGTCCTTAGGGGATAATTTTGTTTTGGC
  TCAGTTGTTCCCTGCTTCCTTTTTTTTTTTTTTTTTTTGAGATGGAGTCTCGCTCTGTTGCCCAGGCTG
  GAGTGCAGTGGTGCGATCTCAGCTCACTGCATCCTCTGCCTCCCGGGTTCAAGCAATTCTCTGCCTCAG
  CCTCCCAAGTAGTTGGGATTACAGGCACCTGCCACCATGCCTGGCTAATTTTTTGTATTTTTAGTAGAG
  ACGGGGGTTTCACTATGTTGGCTAGGCTGGTCTTGAACTCCTGACCTCGTGATCCACCCGCCTTGGCCT
  CCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCACCTGGCCTTCCCTGCTTCCTCTCTAGAATCCAA
  TTAGGGATGTTTGTTACTACTCATATTGATTAAAACAGTTAACAAACTTTTTTCTTTTTAAAATGTGAG
  ATCAGTGAACTCTGGTTTTAAGATAATCTGAAACAAGGTCCTTGGGAGTAATAAAATTGGTCACATTCT
  GTAAAGCACATTCTGTTTAGGAATCAACTTATCTCAAATTGTAACTCGGGGCCTAACTATATGAGATGG
  CTGAAAAAATACCACATCGTCTGTTTTCACTAGGTGATGCCAAAATATTTTGCTTTATGTATATTACAG
  TTCTTTTTAAAACACTGGAAGACTCATGTTAAACTCTAATTGTGAAGGCAGAATCTCTGCTAATTTTTC
  AGATTAAAATTCTCTTTGAAAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000786.4
• Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
• Locus ID: 1595
• MW: 59.9