POLR1B (NM_019014) Human 3' UTR Clone

CAT#: SC214609

3' UTR clone of polymerase (RNA) I polypeptide B 128kDa (POLR1B) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: POLR1B
• Synonyms: A135; RPA2; RPA135; Rpo1-2; TCS4
• ACCN: NM_019014
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC214609 3’UTR clone of NM_019014
  The sequence shown below is from the reference sequence of NM_019014. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AACATCAAAGTGAAACTGGATGTTGTTTAACTTGATGTTGACCTTTTGGATTAAGAGGACTATCAGATT
  AAAGCAAAATGTAATTTTAATTCAATGAAGATATCATTACCAGGTTACTCTTGAGATTTTTCAACGGTG
  TTAGAACTCTCAACCAAGACCTGAAAACCAAGTATGCAAGGTTTCTGAATCTCTCTGGTAGATTAACTA
  TTGACAATGATTTTCTGTTATCTTTGTTCAAAAAGTTCATGTCTTCTCAAAATATGAAATATTGATAAA
  TGGAAGAGCATACGGTGACAAGTCTCCTTTCCAACCCCAGGTTCCCTACACCCTGCTCTCAGCAGGCAG
  TGAGTGTCACACACCTGTTAATCCATCTTGAGCAGGACAGTACTATACAAATAGAATGCAAGCTGTAAT
  GTAATTTTATATTTTCTTATAGCCACGTTGAAGTAAAAACAAACAGGTACAGTGTTTTTTACCAGCTTT
  ATAGAAGTACAGTTGTTACATATTTAATGAATACAATTTGATGGGTCTGACTATATGCACACACCTTTG
  ATACCATCACCACAATCAGGGTAATAAACATACCTGTCATCTCCACAAGTTTCCTCCTGCCCCTTTGTT
  TTTTGCTTTTTGGTTGCTGTTGAGTTTTTGTTTTGTCTTCTGTGGTAAGAACACTTAACTCAAGACCTA
  CCCTCTTAACAAATCTTTAAGTGCACGATATAGTATTGTTAATTCCAGGCACCATGTTGTACAACAGAT
  CTTTAGACCTTACTTGTCTTGCATAACTGAAGCTTTATACCTGTTGAACAACTCTCCATTTCCCTGGCC
  CCTAGCAACCACCCTTCTACCCTGTTTCTATGAGTTTGACTATTACAGATATCTCATATAGTGGGATCA
  TGCAATATTTGTCCTGTGACTGGCTTATTTCACTTAGCATAGTGAAATAAGATTCATCCATTTTGGAAG
  CCAGGCATGGTGCTGTGCATCTATAGTCCCTGCTATTTGAGAGGCTGAGGTGGGAGGATCATTTGAGTG
  CAGGAGTTCAAGGACAGCCTGGGTAATATAGGAAGACCCTGTCTTGAAGACCCTGACCTCAAGTGATCC
  ACCCACCTCGGCCTCCGAAAGTGCTAGGATTACAGGTGTGAGCCACTGTGCCTGGCCTCCGGTGAGTAT
  TTTATATTTAGTCTACACTTCCATACTTGGCTTTTTTCTGCTTTTATATTGATCTGCTTTCATAGCAGT
  GTGTAGAGTGCCACTTATGTTTTCTTTCTTGTGTACAGTATTTTATTGTATGGATTTACCATCCCCTGT
  GTATTTAAGTTGTTCCATTCTTTGGCCATTATAACTTTTTTCTGCAAATATTCTGGTGACTTATCTTTG
  GCCATTATAAACTGTTGATAATAGATCATCTTGTATATACTTCTGCAATTATAAGATGTTTTTTGATGA
  TGAAAGCTTTCCTAAGGTAATTCTTTCCTGAGTTTGGTTCAGATGGTCTGTCACTAATTATAAGGCAGA
  TAGGAACAGACAGAAAAATCTATCATTTCAAGATAGGCTTAGCAGGATGGGCGCAGTGGCTCACGCCAC
  TAATCCCAACATTTTAGGAGGCCTAGGCAGGAGCAATCACTTGTGCCTGGGAGTTCTAGACTAGCCTGG
  GCGAGACTTCATCTCTACAAAAAAAGCAACAACGACAAAAAAAATTAGCCAAGCATAGTGGCACACCCC
  TGTAGTCCCAGCTACTTGGGAGGCTGAGGTGGGAGGATTGCTTGAACCCAAGAGGTCGAGGCTGCAGTG
  AGCCAAGATTGTGCCACTGCACTCCAGCCTGGGTGACAGAGCAGGACCCTGTCTCTATTTTATAAATTA
  AAAAAGGCTGGGTGTGGTGGCTCACACCCATAATCCCAACACTTTGGCTCAGCAGATTGCTTGAACCCA
  GGAATTCAAGTCCAATCTGGGCAACATGGGGAAACCCCAGCTCTACAAAAAAAATTAGCCTGGTGTGG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_019014.6
• Summary: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008]
• Locus ID: 84172
• MW: 74.7