ALDH4A1 (NM_003748) Human 3' UTR Clone

CAT#: SC214551

3' UTR clone of aldehyde dehydrogenase 4 family member A1 (ALDH4A1) nuclear gene encoding mitochondrial protein transcript variant P5CDhL for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ALDH4A1
• Synonyms: ALDH4; P5CD; P5CDh
• ACCN: NM_003748
• Insert Size: 1445 bp
• Sequence Data:
  Insert Sequence

  >SC214551 3’UTR clone of NM_003748
  The sequence shown below is from the reference sequence of NM_003748. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGGGACTGGAGCTACGCGTACATGCAGTGAGCCCCTCTCGGGCTCCACCGTCCAGCTGTCTGTCCGTCC
  AGGTGGCCGACCTCACTGCACAGACCCCACTCCAGCCCCTCCACCCCTTCTTCATGCACAGCTGCCTTT
  CTATAATCCGGGCTTGACTCCCTTCTTACCACTGTATTCTGGCCTCTCCCATGCCTCAGGCTCTGGTTT
  GAGATCGTGCTGGGGAGGAACATGGCCACTACCCCTTATCCCATCGGCCATGTGGGAGGTATGACCCTG
  GTGCCTGGCAGGTTCTCCCTCTGCCCTCCACTGGGCCCAGTGGCTCAGGGACCTGGGGAAAGGAGATGG
  AGCAGCTCTTGGGATCCTTTGGGGAAAAGGAGGCCATTCTGGGCCCCTTGGCAAACCTCACCACTCACA
  GAGGCTCCTGGCCTTGATCCCTGCCCCTCCAGGTGTCCAGGGTAAAGTGTAACTCAGACTGACCTGTGG
  GGCACAGGGGGCACCAGCTGGCCTTGCCCTCTCTGGTCTGGGCTGTCTACCTTCCTCACTGTATCTTTG
  CCCAGACCCACCTGGGCCAGTAGGCCCCTGTCCCCAGCCACACACCTTAGATGCTGGCATGCCTTACTC
  CAGGTGCCTGTGTTTGGCCGAGGCCTGTGTGATTCCCGGTCTGCACCACATGGCGGGGTTGGGGGGCCG
  CTGGAGGCCACCTGCCAAGGCGTGGGATGGGATGGTCCTGCCGGTTTAGGCCGTGATTCTGGAAAACCT
  TGGATGGGCCTTCGTCCTATGTCAGCCTTCCCTTTGATCCTCAGGCCCTACCTGTAGAGACCTCCACTC
  CTAGAGCCAGTCTCAGGGTCTGGGATTTCCCTGCAGGAGCTCAGCCACCACTGTGCCATGGTGACACAG
  GCCAAGGCAGACATTGGCCCTCCCTTCTCCCAGCCCCCAGAGGCCTGGCCTTGGGTTCGTCAGCATGGG
  CCGAGGACGTTGCCTGTAGAATCCTCCTCTGCCTGGGAGTGGCTCTGTGTGGACCAGTCCCTCACTGGC
  CCATTCTTTTTTTGACGCAGCCAATCTGTGACCACGATTCCTCCCACAGATGCCTCCTGCTTGGATTCT
  GAGTGGTCAGAGATCTGTAAAGCATGACTTTCAAGGATGGTTCTTAGGGGACTGTGAAAGTGTTGGGTC
  TTCCTCCAGGATGCCTGCATGGGACCCCACCCGGAGCTGGTGTGGCCATTCCCCAAGTGCCACTGGCCC
  ATGGATGGGGGTGGGTGCTGGTGCCAGCTGGGCTGGGTGTGGGTTCTGTGTCCTTCCAGGATATGTGTC
  ATTTCCCATGAGGGGCCGGGGCAGGTGGCTGGGTGGGGGCACAGGCTGGAGTATTCTTAGTTCTACTGG
  TTCTACACTGTGAGGTGGCAATGGGATTTGCTCAGATGCCACCCAATAAAATGCCTGTTACTTAA
  AGCGGACCGACTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCC
  CAACCTGCCATCACGAGATTTCGATTCCACCGCCGC
  
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003748.4
• Summary: This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2009]
• Locus ID: 8659
• MW: 53.3