RCCD1 (NM_033544) Human 3' UTR Clone

CAT#: SC214158

3' UTR clone of RCC1 domain containing 1 (RCCD1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RCCD1
• ACCN: NM_033544
• Insert Size: 1386 bp
• Sequence Data:
  Insert Sequence

  >SC214158 3’UTR clone of NM_033544
  The sequence shown below is from the reference sequence of NM_033544. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GTGTATGCTGTGGAGAAAGGGAAGAGCTGACATGTGTACGTATATGTATATGCAACACCTGTGAGACCC
  CCATTCAGGTCAAGGAAAACCATTGCCTGCACCCCAAGGGCCCCATATTTGCCCCTCCCCATCACAGTC
  CTGCCCTTCACCCTCAAGCACGGTCCTAAACTTGTCTGCACTTTAGAAACACCTGGAGAGCATTGAAAA
  CTCTGCTGCCTAAGGTCAGCATCAATCAAAACAATGAAATCAATGAAACAATGAAACCAGAGCTTCTAG
  GTGTGTGGCCTGGATAGTGGTAGATTCAAAGCTCCACCCACCTCATCCCAGGTACATTTGATGTGCAGC
  TGAGATTGGGGCACATTCCAGGTCATCGTCTTCTGTCAGTCATTTCCCTGCTTTCGTTTGAATTTTACC
  ATTCTTGTATGCAGCCTTATACGACATAGTTGTGTTTGCTTTTGAGTGTTAGATAAATGGAATCCTGTG
  TATGTGCTTTTGTGTCGTTTTTGTCACTCAGCATTGTATAATCCATCCTTGTTGGGTCCTGGAGTTCAT
  TTTTAATGCTATATGGTGTTCTGTTGTATGAATAATACCTCAAGTGATACAGCCTTGTTGATGGTCATT
  TTTATTGTTTCTGATTTTGGTCATTCCACATAATTCTTTGTGCAGTCTTCTGCACATGTGCACAAGTTC
  CTTTGCAGAACATTGAGTTGAATTGCTAAGTCAGGAGATACCTTTAATTTTACTAGATAACTGTTGCCC
  CCAGCAGGGTGGGGTCTACTGTGGCTAAAATGTCAGGTAGATGCACACTGGTTTTAATTGGCATTTCCC
  TGGATACTGATGAGGTTGAGTAGCTTTTCATTTGTTTTGGATGGCTGGGCATGGTGGCTCACACCTGTA
  GTCCCAGCACTTTGGGAGGCTAAGCAGGGAGATTGCTTGAGGCGAAGAGTTCATATGTTTATTGGCCAC
  TTGGAGTTTTTCTGTTGTCAAGTGCCTGTTCAAGTTTTCTGGCCTTTTAAAATGTTCTGTATTTTCCTT
  AGTGATTTTTCTTTTGGATATTTCAGATACAAGTCTTTTGAGAGTTAGTATTCAGAGTATCTTCTGCTA
  CAATGTAGTTTGTTTCAGTCTCTCTTGGTATTACCAATTTTAATACTTAGGTGATGCTTACTCCATGCC
  AGGTAAGGCTCAAAGTGCTTTACAAGTATCAACTTATTTTGATAAAATAAGCTTATCAGTAGTTTTCTT
  TATAGTTAAATTCTATTTGTGCATGTGTGTGTCTGTGGTTTAGCAAATGTTTCCCACCCATGGGGTCTT
  TTTTATTGTGGTAAAATATACTGAACATAAAATTTATGTTCAATAAAAAACAAAAATTGTTTTTAACCA
  TTTTAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_033544.3
• Summary: Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with KDM8 (PubMed:24981860). Possibly together with KDM8, is involved in proper mitotic spindle organization and chromosome segregation (PubMed:24981860). Plays a role in regulating alpha-tubulin deacetylation and cytoskeletal microtubule stability, thereby promoting cell migration and TGF-beta-induced epithelial to mesenchymal transition (EMT), potentially through the inhibition of KDM8 (PubMed:28455245).[UniProtKB/Swiss-Prot Function]
• Locus ID: 91433
• MW: 53