SKP1 (NM_170679) Human 3' UTR Clone

CAT#: SC214133

3' UTR clone of S-phase kinase-associated protein 1 (SKP1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SKP1
• Synonyms: EMC19; OCP-II; OCP2; p19A; SKP1A; TCEB1L
• ACCN: NM_170679
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC214133 3’UTR clone of NM_170679
  The sequence shown below is from the reference sequence of NM_170679. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAAGAGAACCAGTGGTGTGAAGAGAAGTGAAATGTTGTGCCTGACACTGTAACACTGTAAGGATTGTTC
  CAAATACTAGTTGCACTGCTCTGTTTATAATTGTTAATATTAGACAAACAGTAGACAAATGCAGCAGCA
  AGTCAATTGTATTAGCAGAATATTGTCCTCATTGCATGTGTAGTTTGAGCACAGATCCCAAACCTTACG
  GCCAAGTTTCTTCTAGTATGATGGAAAGTTTCTTTTTTCTTTGCTCTGAATAAAACTGAACTGTGGGTT
  CTCTATAAGTGGCATTTTGGGCTTTCCCTCTTTTTTGTAAAGCAATGTCTGCCTAGTTTATTGTCCAGT
  TAACTTTAGTGACCTTTTAAAAGTTGGCATTGTAAATAAAACAACTTGCAAAAAAGTTTTCTGGAATAG
  AATTAACAAAATATTATCTTTATTCATGAGTTGGAAACTGGAAAAAGGCTTCTTGAAGTAAATGTTCTG
  AGTGGAGCTACTAGGATGTCTTCCAGCCTCCTGCAGTCAAGGAGTACCACTGTATTGATTAGCCTGTAT
  GTAGCAGGGCTCCCTTCATTGCATCTGAGGACTTGTTTTCTTTTTCTTTATTTTTAATCCTCTTAGTTT
  TAAATATATTGCCTAGAGACTCAGTTACTACCCAGTTTGTGGTTTTTTGGGAGAAATGTAACTGGACAG
  TTAGCTTTTCAATTAAAAAGACACTTAACCCATGTGGGATGTCATCTTTTTATAATTAGTGTTCCCATG
  TGGAGAAAATTATTCACACTACTTGCATGTAAAGAATAATTTAACTTTTAACATTAAAATATGTGGTAA
  AACCCAGAAAGCATCCATCATGAATGCAAGATACTTTCAATAAAAAGTAAGTTATATAGTAGGTAGTTA
  AGTTTGCTTTTGTGGACTTAAATGTGTCTCTTCACTTAAATGGGTTGAATGTGTATATATTTGTTCAGC
  TTGAAAAGACTTAGTTTATATCCTAGCTCACTGGAGGCTGCTGACATAACCATAACTTCTGTCCCTTCT
  AATTGTCATTTATATGCCTAACTGGAGCTAGTACTTTAATTCTTAACACAAAATTACTCTGCCATTGTT
  TCCAGCTTCCCTCCTACAATAGAATGAAGTTTTTTTGATGGCTTGAGATGGCTCACAAATTTTGATTTT
  TTTTTCTTCCTTGTGCTCCCTTTTTTTCTCCTTGCTTTTCCAGTTAACATCTATATTCACATGTAATCT
  TGTTTTCTCTTCACATTCACTGAGTTGTTCAGGCTCAGATCATCCCTTGACAGTAGTTTGCCTTCATCT
  CACCTTTCATTTGTCCCAAATTCACCTTATTTAATAAAGTCCCATATGTTGTCTCACTTAATTCCAATT
  TGTTGTCTGTACCAGAGATAGGAAACTATAATGTGTGGGTCAAATCAGGCCCATAGCCTGTTTTTGTAG
  TCTTTGAGCTAAAAATGGTTTTTACGTTGTAAAGAGTTATTTAAAATTCTCTCTCTCTCTCTCTCTCTC
  TCTCAGGAGATAAGCTTGTTTTCTGTTTGTCCTGGTTTTAGTGGGGAAGGTAGCGGTGTATAGTCCTAG
  CTGAATTGTCTCATCTACCAATTCCTGCTATTATAAGATCAACTTCTGCAAAAACCTTATCCACCTCAA
  GTATCCTTAGCAGCTGGGCATGGTGGCTTATGCCTGTAATCTCAACATTTTGGGAGGACAAGACAGGAG
  GATCGTTTGAGCCCAGGAGTTCAAGACCAGCCTGGGCAACACAGAAAGTCCCTGTCTCTCCAAAAAAAA
  AAAAAAAAATTAGCAGTCATATGGTGCATGCCTATAATCCCAACTACTTGAGAGGCTGAGGTGGGAAGA
  TGGCTTGAGCCTGAGAGGTCGAGGCTGCAGTGAGCTGGCATTGCACCACTGCACTCCAGTCTAGGCAAT
  AGAGCGAGACCTTGTCTCAAAAACAAACCAGAAGTATCCTTAGCAGTATTATGACAAACAAATCTTCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_170679.3
• Summary: This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq, Jul 2008]
• Locus ID: 6500
• MW: 76.2