SKP1 (NM_170679) Human 3' UTR Clone
CAT#: SC214133
3' UTR clone of S-phase kinase-associated protein 1 (SKP1) transcript variant 2 for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | SKP1 |
Synonyms | EMC19; OCP-II; OCP2; p19A; SKP1A; TCEB1L |
ACCN | NM_170679 |
Insert Size | 2000 bp |
Sequence Data |
>SC214133 3’UTR clone of NM_170679
The sequence shown below is from the reference sequence of NM_170679. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC AAAGAGAACCAGTGGTGTGAAGAGAAGTGAAATGTTGTGCCTGACACTGTAACACTGTAAGGATTGTTC CAAATACTAGTTGCACTGCTCTGTTTATAATTGTTAATATTAGACAAACAGTAGACAAATGCAGCAGCA AGTCAATTGTATTAGCAGAATATTGTCCTCATTGCATGTGTAGTTTGAGCACAGATCCCAAACCTTACG GCCAAGTTTCTTCTAGTATGATGGAAAGTTTCTTTTTTCTTTGCTCTGAATAAAACTGAACTGTGGGTT CTCTATAAGTGGCATTTTGGGCTTTCCCTCTTTTTTGTAAAGCAATGTCTGCCTAGTTTATTGTCCAGT TAACTTTAGTGACCTTTTAAAAGTTGGCATTGTAAATAAAACAACTTGCAAAAAAGTTTTCTGGAATAG AATTAACAAAATATTATCTTTATTCATGAGTTGGAAACTGGAAAAAGGCTTCTTGAAGTAAATGTTCTG AGTGGAGCTACTAGGATGTCTTCCAGCCTCCTGCAGTCAAGGAGTACCACTGTATTGATTAGCCTGTAT GTAGCAGGGCTCCCTTCATTGCATCTGAGGACTTGTTTTCTTTTTCTTTATTTTTAATCCTCTTAGTTT TAAATATATTGCCTAGAGACTCAGTTACTACCCAGTTTGTGGTTTTTTGGGAGAAATGTAACTGGACAG TTAGCTTTTCAATTAAAAAGACACTTAACCCATGTGGGATGTCATCTTTTTATAATTAGTGTTCCCATG TGGAGAAAATTATTCACACTACTTGCATGTAAAGAATAATTTAACTTTTAACATTAAAATATGTGGTAA AACCCAGAAAGCATCCATCATGAATGCAAGATACTTTCAATAAAAAGTAAGTTATATAGTAGGTAGTTA AGTTTGCTTTTGTGGACTTAAATGTGTCTCTTCACTTAAATGGGTTGAATGTGTATATATTTGTTCAGC TTGAAAAGACTTAGTTTATATCCTAGCTCACTGGAGGCTGCTGACATAACCATAACTTCTGTCCCTTCT AATTGTCATTTATATGCCTAACTGGAGCTAGTACTTTAATTCTTAACACAAAATTACTCTGCCATTGTT TCCAGCTTCCCTCCTACAATAGAATGAAGTTTTTTTGATGGCTTGAGATGGCTCACAAATTTTGATTTT TTTTTCTTCCTTGTGCTCCCTTTTTTTCTCCTTGCTTTTCCAGTTAACATCTATATTCACATGTAATCT TGTTTTCTCTTCACATTCACTGAGTTGTTCAGGCTCAGATCATCCCTTGACAGTAGTTTGCCTTCATCT CACCTTTCATTTGTCCCAAATTCACCTTATTTAATAAAGTCCCATATGTTGTCTCACTTAATTCCAATT TGTTGTCTGTACCAGAGATAGGAAACTATAATGTGTGGGTCAAATCAGGCCCATAGCCTGTTTTTGTAG TCTTTGAGCTAAAAATGGTTTTTACGTTGTAAAGAGTTATTTAAAATTCTCTCTCTCTCTCTCTCTCTC TCTCAGGAGATAAGCTTGTTTTCTGTTTGTCCTGGTTTTAGTGGGGAAGGTAGCGGTGTATAGTCCTAG CTGAATTGTCTCATCTACCAATTCCTGCTATTATAAGATCAACTTCTGCAAAAACCTTATCCACCTCAA GTATCCTTAGCAGCTGGGCATGGTGGCTTATGCCTGTAATCTCAACATTTTGGGAGGACAAGACAGGAG GATCGTTTGAGCCCAGGAGTTCAAGACCAGCCTGGGCAACACAGAAAGTCCCTGTCTCTCCAAAAAAAA AAAAAAAAATTAGCAGTCATATGGTGCATGCCTATAATCCCAACTACTTGAGAGGCTGAGGTGGGAAGA TGGCTTGAGCCTGAGAGGTCGAGGCTGCAGTGAGCTGGCATTGCACCACTGCACTCCAGTCTAGGCAAT AGAGCGAGACCTTGTCTCAAAAACAAACCAGAAGTATCCTTAGCAGTATTATGACAAACAAATCTTCA ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_170679.3 |
Summary | This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq, Jul 2008] |
Locus ID | 6500 |
MW | 76.2 |
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