hnRNP K (HNRNPK) (NM_002140) Human 3' UTR Clone

CAT#: SC214044

3' UTR clone of heterogeneous nuclear ribonucleoprotein K (HNRNPK) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HNRNPK
• Synonyms: AUKS; CSBP; HNRPK; TUNP
• ACCN: NM_002140
• Insert Size: 1354 bp
• Sequence Data:
  Insert Sequence

  >SC214044 3’UTR clone of NM_002140
  The sequence shown below is from the reference sequence of NM_002140. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAGCAGTATGCAGATGTTGAAGGATTCTAATGCAAGATATTTTTTCTTTTTTATAGTGTGAAGCAGTAT
  TCTGGAAAGTTTTTCTAAGACTAGTGAAGAACTGAAGGAGTCCTGCATCTTTTTTTTTTTATCTGCTTC
  TGTTTAAAAAGCCAACATTCCTCTGCTTCATAGGTGTTCTGCATTTGAGGTGTAGTGAAATCTTTGCTG
  TTCACCAGATGTAATGTTTTAGTTCCTTACAAACAGGGTTGGGGGGGGGAAGGGCGTGCAAAAACTAAC
  ATTGAAATTTTGAAACAGCAGCAGAGTGAGTGGATTTTATTTTTCGTTATTGTTGGTGGTTTAAAAAAT
  TCCCCCCATGTAATTATTGTGAACACCTTGCTTTGTGGTCACTGTAACATTTGGGGGGTGGGACAGGGA
  GGAAAAGTAACAATAGTCCACATGTCCCTGGCATCTGTTCAGAGCAGTGTGCAGAATGTAATGCTCTTT
  TGTAAGAAACGTTTTATGATTTTTAAAATAAATTTAGTGAACCTATTTTTGGTGGTCATTTTTTTTTTA
  AGACAGTCATTTTAAAATGGTGGCTGAATTTCCCAACCCACCCCCAAACTAAACACTAAGTTTAATTTT
  CAGCTCCTCTGTTGGACATATAAGTGCATCTCTTGTTGGACATAGGCAAAATAACTTGGCAAACTTAGT
  TCTGGTGATTTCTTGATGGTTTGGAAGTCTATTGCTGGGAAGAAATTCCATCATACATATTCATGCTTA
  TAATAAGCTGGGGATTTTTTGTTTGTTTTTGCAAATGCTTGCCCCTACTTTTCAACAATTTTCTATGTT
  AGTTGTGAAGAACTAAGGTGGGGAGCAGTACTACAAGTTGAGTAATGGTATGAGTATATACCAGAATTC
  TGATTGGCAGCAAGTTTTATTAATCAGAATAACACTTGGTTATGGAAGTGACTAATGCTGAAAAAATTG
  ATTATTTTTATTAGATAATTTCTCACCTATAGACTTAAACTGTCAATTTGCTCTAGTGTCTTATTAGTT
  AAACTTTGTAAAATATATATATACTTGTTTTTCCATTGTATGCAAATTGAAAGAAAAAGATGTACCATT
  TCTCTGTTGTATGTTGGATTATGTAGGAAATGTTTGTGTACAATTCAAAAAAAAAAAAGATGAAAAAAG
  TTCCTGTGGATGTTTTGTGTAGTATCTTGGCATTTGTATTGATAGTTAAAATTCACTTCCAAATAAATA
  AAACACCCATGATGCTAGATTTGATGTGTGCCCGATTTGAACAAGGGTTGATTGACACCTGTAAAATTT
  GTTGAAACGTTCCTCTTAAAAGGAAATATAGTAATCTTATGTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002140.5
• Summary: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]
• Locus ID: 3190
• MW: 52.4