AGBL4 (NM_032785) Human 3' UTR Clone

CAT#: SC213893

3' UTR clone of ATP/GTP binding protein-like 4 (AGBL4) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AGBL4
• Synonyms: CCP6
• ACCN: NM_032785
• Insert Size: 1349 bp
• Sequence Data:
  Insert Sequence

  >SC213893 3’UTR clone of NM_032785
  The sequence shown below is from the reference sequence of NM_032785. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AACCACAAAGACCCTTCAACCCCTTTTTAAAGACACGGAGTCCTGGGAGGTCTTATGGGGACAGGAGCA
  TGCATTTTCTGCTGGGGAATGAAATTAATCACTCTTCTCTAGCTTCCAAGGATTTGTGGATTAATGCAT
  AGGCTAGTGAAAGGGAAGGGATACAATGAGAAACTTCATCACTGATTTTCCCTTTTTCCAATGGAAAAT
  CAATTGTAAGCCTTCAGCTCAAAAAGCTCAGGAACAAAATAGGAGATCGGAACCAGGTGAGCACAGATG
  CTATTTTCTACAACATAGAGATCTTCCAAGATGAGGAAAACCTACACATCCCTTTTAAGAGATTTGACA
  CATATCAGGTCAGTTCTTTCAAAGCCACTTTACATTTTTAAGTTTTATAGTTACTGCAGATGGTTACCT
  TTTAAATAGGCTTGGTTGTTTACAGCTTTAGAAATGCAGTGTTTGTATTTTCTGATCCAAAACAACAGC
  AACAACACATGCAACCAAAGGGCAAATGCATCCTAGGGAACAATGGGACAGAATTTTTGAAATCAACGG
  TGGACTTAAATATATGGTCACTGTATCTAAGCTGCATGCTTGCTGATTCCCTCAAACCCCAGCATGCAG
  ATTTAGAAAACTGTTTTGAGAGCTGTTTCCCAATTTCCTCGGTAGTTTAATTAAAATTTTAGCTCTAAA
  GACAAAAAGCACTGGTCACTCATTTTAAAAGGTTTATTATGGTTAAGGTCTCCAGATCCCAGGTGACAG
  GGATTAAGCCTGGCCCCTGAAGATGTAGGCATTGCTAATCTGCAATGAGGAAAGGGTGATTGCCAGGCA
  AGGAAGATGCAAAAGACAGAAGGATGCACCCTGAGGCAGGGAGGAATGATAAAATAAAACAGTAGTAGC
  AATTCCCAAGGTGTGGCAAGCTAAATATTGGCATTTTTGTGAAGAGTAAAGTGCCTTTGTATGATGAAT
  GGGTCCAGGGGGGTCTGCTCCCTTCCTGTCTGTGGAAACAGCAGGCTGAGTCACCATGACAGCCTGCTA
  ATTAAAATGACCTCCCGGAAATGAGGATTAGAACACAGGGAGAGGAAGGGAGGCTCCAGGGAGGAAAAT
  CCCTTAGCACAGAGCTCCACTAAAGCATTTTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGAGTTGGA
  GATTATTGAAGTTTGCTACAAAAATCAAAAAGAAAACTTGTTCTCCTTTGCATGCTAGTTTTCAGAAAT
  GATGACAAAGAGCCATATGTTGATTGGCTACAAATGTCATGTTGTATATACAGCTTCTCCCCTGGGTTC
  GTTTTGCCCAGAGGAACACAAATAAAGTGTTTTGTGCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_032785.4
• Summary: Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes polyglutamates from the carboxy-terminus of target proteins such as MYLK. Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5.[UniProtKB/Swiss-Prot Function]
• Locus ID: 84871
• MW: 50.9