PLOD2 (NM_000935) Human 3' UTR Clone
CAT#: SC213728
3' UTR clone of procollagen-lysine 2-oxoglutarate 5-dioxygenase 2 (PLOD2) transcript variant 2 for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | PLOD2 |
Synonyms | BRKS2; LH2; TLH |
ACCN | NM_000935 |
Insert Size | 1307 bp |
Sequence Data |
>SC213728 3’UTR clone of NM_000935
The sequence shown below is from the reference sequence of NM_000935. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC TACATTGCAGTGTCATTTATAGATCCCTAAGTTATTTACTTTTCATTGAATTGAAATTTATTTTGGATG AATGACTGGCATGAACACGTCTTTGAAGTTGTGGCTGAGAAGATGAGAGGAATATTTAAATAACATCAA CAGAACAACTTCACTTTGGGCCAAACATTTGAAAAACTTTTTATAAAAAATTGTTTGATATTTCTTAAT GTCTGCTCTGAGCCTTAAAACACAGATTGAAGAAGAAAAGAAAGAAAAAACTTAAATATTTATTTCTAT GCTTTGTTGCCTCTGAGAATAATGACAATTTATGAATTTGTGTTTCAAATTGATAAAATATTTAGGTAC AAATAACAAGACTAATAATATTTTCTTATTTAAAAAAAGCATGGGAAGATTTTTATTTATCAAAATATA GAGGAAATGTAGACAAAATGGATATAAATGAAAATTACCATGTTGTAAAACCTTGAAAATCAGATTCTA ACTGGATTTGTATGCAACTAAGTATTTTTCTGAACACCTATGCAGGTCTTATTTACAGTAGTTACTAAG GGAACACACAAAGAATTACACAACGTTTTCCTCAAGAAAATGGTACAAAACACAACCGAGGAGCGTATA CAGTTGAAAACATTTTTGTTTTGATTGGAAGGCAGATTATTTTATATTAGTATTAAAAATCAAACCCTA TGTTTCTTTCAGATGAATCTTCCAAAGTGGATTATATTAAGCAGGTATTAGATTTAGGAAAACCTTTCC ATTTCTTAAAGTATTATCAAGTGTCAAGATCAGCAAGTGTCCTTAAGTCAAACAGGTTTTTTTTGTTGT TGTTTTTGCTTTGTTTCCTTTTTTAGAAAGTTCTAGAAAATAGGAAAACGAAAAATTTCATTGAGATGA GTAGTGCATTTAATTATTTTTTAAAAAACTTTTTAAGTACTTGAATTTTATATCAGGAAAACAAAGTTG TTGAGCCTTGCTTCTTCCGTTTTGCCCTTTGTCTCGCTCCTTATTCTTTTTTTGGGGGGAGGGTTATTT GCTTTTTTATCTTCCTGGCATAATTTCCATTTTATTCTTCTGAGTGTCTATGTTAACTTCCCTCTATCC CGCTTATAAAAAAATTCTCCAACAAAAATACTTGTTGACTTGATGTTTTATCACTTCTCTAAGTAAGGT TGAAATATCCTTATTGTAGCTACTGTTTTTAATGTAAAGGTTAAACTTGAAAAGAAATTCTTAATCACG GTGCCAAAATTCATTTTCTAACACCATGTGTTAGAAAATTATAAAAAATAAAATAATTTTAGAAA ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_000935.3 |
Summary | The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Locus ID | 5352 |
MW | 52 |
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