UPP2 (NM_173355) Human 3' UTR Clone

CAT#: SC213551

3' UTR clone of uridine phosphorylase 2 (UPP2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: UPP2
• Synonyms: UDRPASE2; UP2; UPASE2
• ACCN: NM_173355
• Insert Size: 1294 bp
• Sequence Data:
  Insert Sequence

  >SC213551 3’UTR clone of NM_173355
  The sequence shown below is from the reference sequence of NM_173355. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ATCAGACGGCGGCTTGGACTTTGTGACTAGACGTCCTAACTGGGCAGCCCAACCCTCCCCTGCAAGTTT
  GTAGCTCAAGTTGTAATGTGAAAGTCATATTTTATTTGTGGCATTTTTATATAGTTCTCATCCACATGC
  TAAATGGAAAGACTTTATGAAATCCTTCTCTCTTAAAAAGGAATTTATTGTAAAAGAATACTCACACTA
  AATTAAATTCAAATTTCATTTTAGAATAAGTTAACTAAATCAGTCTAATAATTAAAATTTTAAAACTCC
  TGGATTATGACATTTGGAGTTTCATATGCAGCATTAATTAAGCTCACATCAAAATAGACCAGCCATTTG
  CACATAGTTACCAGTCACTCTGTTTCTGAAACCAATCCAGAAATTCATGTTAGAACATTGTCAGGCACT
  TCTAGGTATTGGACAAGTGACAGAAACTGATCATCCATATTCAAAATATTTACAGAAAAAAGTGTCAGT
  GAAATGTAGGTCTATATGGAAAAGTTACCATTAGGAAAATATGGCCATACTGCTTTTTTACCCCTGCAC
  TATTTCAGTGCACCACAGAATAGGATTCGAGATTTACATATGCCTGTTTGTGATTTTATCTCAGAATAT
  AACAACTCTGGATTACATTTTCCTCTCAACTGAAAACCCTCAGTAATGCAAAGAAAGGCCTTCCTTTTT
  CTTCAGAATCACAAGTGACTAAATTGCCTTCTAGCCATTTTTTCTTGTAAAAAAAGTGTTTTGAGGAGG
  CTGTATTTTTTATTCCATTTCAATATTAACCAAATAGGCTGAGAAATTCATAGCAATTGAATTAAGACT
  TGAGTTCAAGATGTTAAAGGAATCCTTTTGTATGACTTGTCCAGACCGGCCTATTGCCCTACCCGTTTG
  ATTCTCTGGGAGGCATGGTGCTCCTCCTGGCTGCACATGCTCTCTGTGAACTGATCGCATCTTGGGTTC
  TTCTCAATTTGACATACTTGGGAGTCGGAATGACTGGATGGGGGATGGGTAGAGGATGACTGGCACACT
  TGTATAGTATGTAATGTTGTGTACATATTATTTATATGGTTGTGGCTCAAATAAAGTTTAATTCCAACA
  CAGTACAGATTATTTGGATCCCAGAGCCATTGTCACACATGACCGAATGGAGGCTTGGGGCAGGAAATA
  GGTCCTAGGCCGGTTGAGTCTGAATTGGCCTGCTCCTGGGTGGGGAATGTCTTTCTCTTTCAAGCAATG
  ACAATAAACAAGAGGTATTTATCATCACCAATAAACAATCCCCCAAGAGGTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_173355.4
• Summary: Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. Shows substrate specificity and accept uridine, deoxyuridine, and thymidine as well as the two pyrimidine nucleoside analogs 5-fluorouridine and 5-fluoro-2(')-deoxyuridine as substrates.[UniProtKB/Swiss-Prot Function]
• Locus ID: 151531
• MW: 49.7