COMT (NM_000754) Human 3' UTR Clone

CAT#: SC213426

3' UTR clone of catechol-O-methyltransferase (COMT) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: COMT
• Synonyms: HEL-S-98n
• ACCN: NM_000754
• Insert Size: 1269 bp
• Sequence Data:
  Insert Sequence

  >SC213426 3’UTR clone of NM_000754
  The sequence shown below is from the reference sequence of NM_000754. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAGGGCCCAGGCAGCGAAGCAGGGCCCTGACTGCCCCCCCGGCCCCCCTCTCGGGCTCTCTCACCCAGC
  CTGGTACTGAAGGTGCCAGACGTGCTCCTGCTGACCTTCTGCGGCTCCGGGCTGTGTCCTAAATGCAAA
  GCACACCTCGGCCGAGGCCTGCGCCCTGACATGCTAACCTCTCTGAACTGCAACACTGGATTGTTCTTT
  TTTAAGACTCAATCATGACTTCTTTACTAACACTGGCTAGCTATATTATCTTATATACTAATATCATGT
  TTTAAAAATATAAAATAGAAATTAAGAATCTAAATATTTAGATATAACTCGACTTAGTACATCCTTCTC
  AACTGCCATTCCCCTGCTGCCCTTGACTTGGGCACCAAACATTCAAAGCTCCCCTTGACGGACGCTAAC
  GCTAAGGGCGGGGCCCCTAGCTGGCTGGGTTCTGGGTGGCACGCCTGGCCCACTGGCCTCCCAGCCACA
  GTGGTGCAGAGGTCAGCCCTCCTGCAGCTAGGCCAGGGGCACCTGTTAGCCCCATGGGGACGACTGCCG
  GCCTGGGAAACGAAGAGGAGTCAGCCAGCATTCACACCTTTCTGACCAAGCAGGCGCTGGGGACAGGTG
  GACCCCGCAGCAGCACCAGCCCCTCTGGGCCCCATGTGGCACAGAGTGGAAGCATCTCCTTCCCTACTC
  CCCACTGGGCCTTGCTTACAGAAGAGGCAATGGCTCAGACCAGCTCCCGCATCCCTGTAGTTGCCTCCC
  TGGCCCATGAGTGAGGATGCAGTGCTGGTTTCTGCCCACCTACACCTAGAGCTGTCCCCATCTCCTCCA
  AGGGGTCAGACTGCTAGCCACCTCAGAGGCTCCAAGGGCCCAGTTCCCAGGCCCAGGACAGGAATCAAC
  CCTGTGCTAGCTGAGTTCACCTGCACCGAGACCAGCCCCTAGCCAAGATTCTACTCCTGGGCTCAAGGC
  CTGGCTAGCCCCCAGCCAGCCCACTCCTATGGATAGACAGACCAGTGAGCCCAAGTGGACAAGTTTGGG
  GCCACCCAGGGACCAGAAACAGAGCCTCTGCAGGACACAGCAGATGGGCACCTGGGACCACCTCCACCC
  AGGGCCCTGCCCCAGACGCGCAGAGGCCCGACACAAGGGAGAAGCCAGCCACTTGTGCCAGACCTGAGT
  GGCAGAAAGCAAAAAGTTCCTTTGCTGCTTTAATTTTTAAATTTTCTTACAAAAATTTAGGTGTTTACC
  AATAGTCTTATTTTGGCTTATTTTTAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000754.4
• Summary: Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]
• Locus ID: 1312
• MW: 46