LEF1 (NM_001130713) Human 3' UTR Clone

CAT#: SC213362

3' UTR clone of lymphoid enhancer-binding factor 1 (LEF1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: LEF1
• Synonyms: LEF-1; TCF1ALPHA; TCF7L3; TCF10
• ACCN: NM_001130713
• Insert Size: 1240 bp
• Sequence Data:
  Insert Sequence

  >SC213362 3’UTR clone of NM_001130713
  The sequence shown below is from the reference sequence of NM_001130713. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGTCCAAGAATGACAGCTGCCTACATCTGAAACATGGTGGAAAACGAAGCTCATTCCCAACGTGCAAAG
  CCAAGGCAGCGACCCCAGGACCTCTTCTGGAGATGGAAGCTTGTTGAAAACCCAGACTGTCTCCACGGC
  CTGCCCAGTCGACCCCAAAGGAACACTGACATCAATTTTACCCTGAGGTCACTGCTAGAGACGCTGATC
  CATAAAGACAATCACTGCCAACCCCTCTTTCGTCTACTGCAAGAGCCAAGTTCCAAAATAAAGCATAAA
  AAGGTTTTTTAAAAGGAAATGTAAAAGCACATGAGAATGCTAGCAGGCTGTGGGGCAGCTGAGCAGCTT
  TTCTCCCCTCATATCTGCGTGCACTTCCCAGAGCATCTTGCATCCAAACCTGTAACCTTTCGGCAAGGA
  CGGTAACTTGGCTGCATTTGCCTGTCATGCGCAACTGGAGCCAGCAACCAGCACATCCATCAGCACCCC
  AGTGGAGGAGTTCATGGAAGAGTTCCCTCTTTGTTTCTGCTTCATTTTTCTTTCTTTTCTTTTCTCCTA
  AAGCTTTTATTTAACAGTGCAAAAGGATCGTTTTTTTTTGCTTTTTTAAACTTGAATTTTTTTAATTTA
  CACTTTTTAGTTTTAATTTTCTTGTATATTTTGCTAGCTATGAGCTTTTAAATAAAATTGAAAGTTCTG
  GAAAAGTTTGAAATAATGACATAAAAAGAAGCCTTCTTTTTCTGAGACAGCTTGTCTGGTAAGTGGCTT
  CTCTGTGAATTGCCTGTAACACATAGTGGCTTCTCCGCCCTTGTAAGGTGTTCAGTAGAGCTAAATAAA
  TGTAATAGCCAAACCCACTCTGTTGGTAGCAATTGGCAGCCCTATTTCAGTTTATTTTTTCTTCTGTTT
  TCTTCTTTTCTTTTTTTAAACAGTAAACCTTAACAGATGCGTTCAGCAGACTGGTTTGCAGTGAATTTT
  CATTTCTTTCCTTATCACCCCCTTGTTGTAAAAAGCCCAGCACTTGAATTGTTATTACTTTAAATGTTC
  TGTATTTGTATCTGTTTTTATTAGCCAATTAGTGGGATTTTATGCCAGTTGTTAAAATGAGCATTGATG
  TACCCATTTTTTAAAAAAGCAAGGCACAGCCTTTGCCCAAAACTGTCATCCTAACGTTTGTCATTCCAG
  TTTGAGTTAATGTGCTGAGCATTTTTTTAAAAGAAGCTTTGTAATAAAACATTTTTAAAAATTGTCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001130713.3
• Summary: This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
• Locus ID: 51176
• MW: 47.9