ROC2 (RNF7) (NM_183237) Human 3' UTR Clone

CAT#: SC213145

3' UTR clone of ring finger protein 7 (RNF7) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RNF7
• Synonyms: CKBBP1; rbx2; ROC2; SAG
• ACCN: NM_183237
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC213145 3’UTR clone of NM_183237
  The sequence shown below is from the reference sequence of NM_183237. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TCCACAACTGCTGCATGTCCCTGTGGGTGAAACAGAACAATCGCTGCCCTCTCTGCCAGCAGGACTGGG
  TGGTCCAAAGAATCGGCAAATGAGAGTGGTTAGAAGGCTTCTTAGCGCAGTTGTTCAGAGCCCTGGTGG
  ATCTTGTAATCCAGTGCCCTACAAAGGCTAGAACACTACAGGGGATGAATTCTTCAAATAGGAGCCGAT
  GGATCTGTGGTCCTTTGGGACTCATCAAAGCCTTGGTTTAGCATTTTGTCAGTTTTATCTTCAGAAATT
  CTCTGCGATTAAGAAGATAATTTATTAAAGGTGGTCCTTCCTACCTCTGTGGTGTGTGTCGCGCACACA
  GCTTAGAAGTGCTATAAAAAAGGAAAGAGCTCCAAATTGAATCACCTTTATAATTTACCCATTTCTATA
  CAACAGGCAGTGGAAGCAGTTTCAGAGAACTTTTTGCATGCTTATGGTTGATCAGTTAAAAAAGAATGT
  TACAGTAACAAATAAAGTGCAGTTTAAAACCCAACTCTTACTCTTAATTTGTTCCTAATACGTATTTTT
  GGCAGGGAGAGGGAACGGTCCATGAAATCTTTATGTGATATAAGGATTTTAAGTTTGGGCCAGTGAACA
  GGGTAAATAAAATTTAACTTTTGAGCATATGGAATTTTGATTGCCTTTAAAGTTACTATTCTGTATCAT
  TGATTCATCAAGAAAACCTAGATCTGCACTCACCTCACTGTTCAATCATTGAGTGGTAAAGGACAGAAG
  TATTTTCTAGTTCTGGTCAGATGATATAATTTTTTTTTTTTTTTTTTGTGGGGGTGAAGTCTTGCTCTG
  CCACCCAGGCTGGAGTGCAGTGGCATGATGTCAACTCACTGCAACCTCCGCCTCCCGGGTTCAAGTGAT
  TCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTGTGTGCCACCACACCCGGCTAATTTTTGTA
  TTTTTAGTAGAGACGGGATTTCCCCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATTCG
  CCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGGGCCACTGCACCTGGCCCAGATGATATAAT
  TTAACTGTGTTTTAGGTAACATGTTAATGAGGGAAAATGTTTACCAGTGTAGCATTGGATCAAAAAGTT
  TATCACACAGTGTTTTAATAAATGGTTTATTCTGCTTACCTTATTTGAATTGTCATTATAATTTTATAT
  GCAACCTTAATTTGTCAAGTAAATACTTTCATTTAATTTGAAAGAATTCATATTCATTTGCTCTTTCAT
  TTGACAATAATAAAATTTAAGGTTTATTACCTGGAAAGAGTGCAAGAAAATACTAAACAACTTTATCAA
  ACTGATTCACACTGAGGGGAGGTTATTTTGTAACAAATAATGGACCACAAGTTCATTTTGTATTCTTCT
  GTAGTAGAGGCTACTGATATGAATTTAAGAAAAAACCTTAGAATCTTAACTTTTCACATCTGCAGATGC
  AGTGTGCCATTCTGAATTTTTCAATCTAAGGGATGTGGATACATAGAATGTTTAAAAAGATAACAATTA
  GAATGAAAAATTAAAATTATGTATGCTTTTATATGGGTTACTTTTTATTGATCACATTTCTCTGGAAAT
  TTTTCTTTATATTTTTTCACAGTTGTCTTTCTAGCTACTGAAAAAATTATACAAATATCTTTGGCCATT
  TAAATGTTTATCATTGTGCAGTGAACTGTTTTAGTAACTTTCTGTTTGACCTTTTTAGAAGGTTGCATC
  CTTAAAATGTGAACCAGATACATGGCTTTGCATTGAAGAGGAAAGCAGTAGAATGTCAGGTTACTTTAG
  GCAGTGTGACAGTATATAAATGAAATGTGGAGGATGGTGGTAACAATTTGATTTTCCACACAGCAAAAC
  CACAGTCCCGCCACCTAACAAAGCCCTGTTTATGCCACACACCAGCATCTGAGGGAAGAGGAGGCACT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_183237.3
• Summary: The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
• Locus ID: 9616
• MW: 75.6