C 4 Methylsterol Oxidase (MSMO1) (NM_006745) Human 3' UTR Clone

CAT#: SC213139

3' UTR clone of sterol-C4-methyl oxidase-like (SC4MOL) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MSMO1
• Synonyms: DESP4; ERG25; MCCPD; SC4MOL
• ACCN: NM_006745
• Insert Size: 1248 bp
• Sequence Data:
  Insert Sequence

  >SC213139 3’UTR clone of NM_006745
  The sequence shown below is from the reference sequence of NM_006745. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGGAAGAAGTTTGAGAAAAAGACTGAATAAATATCTCACGTAAACCTTCCTGAAAGATAAACGTTTTCC
  TGAATTCAGAAACTAGTAGCTAACATTGCTTCTGGAGAGCAGAAATAAGCATGTCTTCTGGCTACTAAG
  TGATAAAAAGAACATTAACAACCTTTAATTACCTTCCTAGTGGGAACTTTTTCTACTTTACCTACAAGT
  TCTATATATGTAGAAATGAATAAATATATATTTAAGTACAGTTTTCATGAGGAAGTTTTAAAAGACCAT
  GTTCCTAAGCTTCCAAGAAGGTTTTGGATACTAGAAGTATTAATCTATGGCTTTTCTCCCAGTAAAACC
  ATAGGCCTGAAGTTCACATTGGGTCTTTAAATCTTTTAGATATATACTGGTCATTTCAGAAAATTCTTC
  ATAGTGGTATTGGCCTTATATTTAACTTTTTTTTTATTTTTTTTTTGAGACAAAGCCACACTCTGTCTC
  CTTGGCTGGAGTGTGGTGGCACAGTCTCAGCTCACTGCAACCTCTGCCTCCCAGTTCAAGCAATTCTTC
  TGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCACCCGCCACCACGCCCAGCTAATTTTTGTATTTTT
  GTAGAGATGGGGTTTCACGATGTTGGCCAGGCTGGTCTCAAACTTCTGACCTCAAGTGATCTGCCCACC
  TTGGCCTCCCAAAGTGCTGGGATTACAGGTGTAAGCCACTGCGCCCGGCCTTTTTAACTTTAAACATGT
  TTTAGAATTCACCTAAAGATCAAAATATCATGGATTGAACCTCATCAATTGATAGCAGTGAGTGACTGA
  AGCTTCCAAATCAAGAAAAGCCGGCACCAAGAACTTCCATTCTAATCTAGAGCTGACCAGTTTGAGCTG
  ATTCTCTCTTTGAAGAGTCCTTCTTGATTGCAGTGCAGTACTGGCATTTCTGAATGGATGTAAGTGGAG
  TATTTTAGTCTAAAGGCTTTTCAAATTACTTGAATTTTTTTAAAAATTGAGGAGCTTTATTTCTATTTA
  CCCTTCCATTTTTGTATATCAAATTTCCATTGTCATTAAAAACTGTATCTTGAAACTTTGTGAACTGAC
  TTGCTGTATTTGCACTTTGAGCTCTTGAAATAAATGTGATTTTTGTGTGATTATCTGGTTTCCAGTTTT
  AAACATTAACTGTCACCTTTTATTCTTAAACTTGAAAGTACAGAAATCATTAAATTATTAAGTTGTACA
  ATAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006745.5
• Summary: Sterol-C4-mehtyl oxidase-like protein was isolated based on its similarity to the yeast ERG25 protein. It contains a set of putative metal binding motifs with similarity to that seen in a family of membrane desaturases-hydroxylases. The protein is localized to the endoplasmic reticulum membrane and is believed to function in cholesterol biosynthesis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 6307
• MW: 48.2