TAF2 (NM_003184) Human 3' UTR Clone

CAT#: SC212977

3' UTR clone of TAF2 RNA polymerase II TATA box binding protein (TBP)-associated factor 150kDa (TAF2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TAF2
• Synonyms: CIF150; MRT40; TAF2B; TAFII150
• ACCN: NM_003184
• Insert Size: 1180 bp
• Sequence Data:
  Insert Sequence

  >SC212977 3’UTR clone of NM_003184
  The sequence shown below is from the reference sequence of NM_003184. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TCTATTCGTTCTCCTTCCCTTTCAGACTGAGAAGGGGACAAAAAGACCTTTCCTTTCATGTCCAGAAGA
  ATGTATGTAACTAAAGCTTTGTCCTCTGTGAAGAATTATAAAAGGGAGGGGGGAAAGGATTCGCCTCTC
  CTACAGAAATTCTGAATTCATTTAAGTTCTAAGCATTTGATTTATGTTATTTATACAGTTGGGATCTAA
  TTAGGAAAATGTGTTTTGTAGTTCTGGATAAACTATTTCATCCGCTGTTTCCTCCCCAAAACACACACA
  CAGAGCAAACTCCCTTTCATAAAAGCCCTCATATCCACTGGCAGTCCCCGTTCGCATCATGGTCTCCAT
  GTGTACCGCCAAAGTCAATTATGTTTGAAAGCCTTTGGTGGATGTTATGGGGCAAAGTTATGATTTACA
  CAGAAGCAACTGCCAAATCTGTGGTGCAACCACTATCTCCAGTGAAATATTGTATAACACCATTTGGAA
  CTACTGAAAAGACAGTGGCTTTTCTACAGTACTCTTCCTTATTGCACCATTTTTGTATTAACGTAGAAA
  CTAAGCATCAGAATTTATGAACAAAGAATATGTTATTTTTCCTTTTGCTCTAAAATACTGAGATTTGGG
  GAAGCAATTCTTTTTTAAAAAAATTTTGAATAACTATCTTTTGATAACACATTCGGCGGTTACGATGTT
  GGAATTTAGCAGAACTATCAAATCCAAAATTATTTTCAAATCAAGATGTTTAAATTATAAGTTTTTTTG
  TTTTGTTTTCCAGTAAGTATAAATGAAAATATTCATATGTAAAATATGTTTTGCTTAATGTGGACAATT
  TACACACCCAACACATACTGTTTCCAAAATTATTTCTATATGACACAAAGTATGATACCTTTAAAAAGG
  TGAGCTTATATATTGTTTATTAACCTGGAAATAGACAATTTGGCTCTCATACCATAAGCATTTAAAAAC
  ATATGAAATATTTTGATTTCTTTATATGGTGGTGTGTTTTTATTTCTGAATTTTCCATGTGCTTTAATA
  TTTGTTTAAGAATATTTTTTATATCTGTTCAATTTTTATCATTCCCCTTATCATTAGAAAGTTTGACAA
  AAATACTTGAATCTTGGACCTCTAAATATACTTTTAGTTGTGTTTAACTTGTTTAATAAATAACTTTTT
  AAGTTGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003184.4
• Summary: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators. [provided by RefSeq, Jul 2008]
• Locus ID: 6873
• MW: 46.5