RPL32 (NM_001007073) Human 3' UTR Clone

CAT#: SC212881

3' UTR clone of ribosomal protein L32 (RPL32) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RPL32
• Synonyms: L32; PP9932
• ACCN: NM_001007073
• Insert Size: 1196 bp
• Sequence Data:
  Insert Sequence

  >SC212881 3’UTR clone of NM_001007073
  The sequence shown below is from the reference sequence of NM_001007073. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GCCAGGCTGCGCAGTGAAGAAAATGAGTAGGCAGCTCATGTGCACGTTTTCTGTTTAAATAAATGTAAA
  AACTGCCATCTGGCATCTTCCTTCCTTGATTTTAAGTCTTCAGCTTCTTGGCCAACTTAGTTTGCCACA
  GAGATTGTTCTTTTGCTTAAGCCCCTTTGGAATCTCCCATTTGGAGGGGATTTGTAAAGGACACTCAGT
  CCTTGAACAGGGGAATGTGGCCTCAAGTGCACAGACTAGCCTTAGTCATCTCCAGTTGAGGCTGGGTAT
  GAGGGGTACAGACTTGGCCCTCACACCAGGTAGGTTCTGAGACACTTGAAGAAGCTTGTGGCTCCCAAG
  CCACAAGTAGTCATTCTTAGCCTTGCTTTTGTAAAGTTAGGTGACAAGTTATTCCATGTGATGCTTGTG
  AGAATTGAGAAAATATGCATGGAAATATCCAGATGAATTTCTTACACAGATTCTTACGGGATGCCTAAA
  TTGCATCCTGTAACTTCTGTCCAAAAAGAACAGGATGATGTACAAATTGCTCTTCCAGGTAATCCACCA
  CGGTTAACTGGAAAAGCACTTTCAGTCTCCTATAACCCTCCCACCAGCTGCTGCTTCAGGTATAATGTT
  ACAGCAGTTTGCCAAGGCGGGGACCTAACTGGTGACAATTGAGCCTCTTGACTGGTACTCAGAATTTAG
  TGACACGTGGTCCTGATTTTTTTTGGAGACGGGGTCTTGCTCTCACCCAGGCTGGGAGTGCAGTGGCAC
  ACTGACTACAGCCTTGACCTCCCCAGGCTCAGGTGATCTTCCCACCTCAGCCTTCCAAGTAGCTGGGAC
  TACAGATGCACACCTCCAAACCTGGGTAGTTTTTGAAGTTTTTTTGTAGAGGTGGTCTAGCCATGTTGC
  CTAGGCTCCCGAACTCCTGAGCTCAAGCAATCCTGCTTCAGCCTCCCAAAGTACTGGGATTACAGGCAT
  CTTCTGTAGTATATAGGTCATGAGGGATATGGGATGTGGTACTTATGAGACAGAAATGCTTACAGGATG
  TTTTTCTGTAACCATCCTGGTCAACTTAGCAGAAATGCTGCGCTGGGTATAATAAAGCTTTTCTACTTC
  TAGTCTAGACAGGAATCTTACAGATTGTCTCCTGTTCAAAACCTAGTCATAAATATTTATAATGCAAAC
  TGGTCAAAAAAAAAAAAAAAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001007073.1
• Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 6161
• MW: 45.1