L1CAM (NM_001143963) Human 3' UTR Clone

CAT#: SC212872

3' UTR clone of L1 cell adhesion molecule (L1CAM) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: L1CAM
• Synonyms: CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1
• ACCN: NM_001143963
• Insert Size: 1179 bp
• Sequence Data:
  Insert Sequence

  >SC212872 3’UTR clone of NM_001143963
  The sequence shown below is from the reference sequence of NM_001143963. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCCATCAACCCTGCCGTGGCCCTAGAATAGTGGAGTCCAGGACAGGAGATGCTGTGCCCCTGGCCTTGG
  GATCCAGGCCCCTCCCTCTCCAGCAGGCCCATGGGAGGCTGGAGTTGGGGCAGAGGAGAACTTGCTGCC
  TCGGATCCCCTTCCTACCACCCGGTCCCCACTTTATTGCCAAAACCCAGCTGCACCCCTTCCTGGGCAC
  ACGCTGCTCTGCCCCAGCTTGGGCAGATCTCCCACATGCCAGGGGCCTTTGGGTGCTGTTTTGCCAGCC
  CATTTGGGCAGAGAGGCTGTGGTTTGGGGGAGAAGAAGTAGGGGTGGCCCGAAAGGGTCTCCGAAATGC
  TGTCTTTCTTGCTCCCTGACTGGGGGCAGACATGGTGGGGTCTCCTCAGGACCAGGGTTGGCACCTTCC
  CCCTCCCCCAGCCACTCCCCAGCCAGCCTGGCTGGGACTGGGAACAGAACTCGGTGTCCCCACCATCTG
  CTGTCTTTTCTTTGCCATCTCTGCTCCAACCGGGATGGGAGCCGGGCAAACTGGCCGCGGGGGCAGGGG
  AGGCCATCTGGAGAGCCCAGAGTCCCCCCACTCCCAGCATCGCACTCTGGCAGCACCGCCTCTTCCCGC
  CGCCCAGCCCACCCCATGGCCGGCTTTCAGGAGCTCCATACACACGCTGCCTTCGGTACCCACCACACA
  ACATCCAAGTGGCCTCCGTCACTACCTGGCTGCGGGGCGGGCACACCTCCTCCCACTGCCCACTGGCCG
  GCCTCTTCCAGCCGCCCCCACCCCCCAGGACCCCTTAGCAGCCCTGCCCTCCCTATCGTCTGAACAGTT
  GTCTTCCTCAGCCTCCTCCCGCCCCCACCTTGGGAATGTAAATACACCGTGACTTTGAAAGTTTGTACC
  CCTGTCCTTCCCTTTACGCCACTAGTGTGTAGGCAGATGTCTGAGTCCCTAGGTGGTTTCTAGGATTGA
  TAGCAATTAGCTTTGATGAACCCATCCCAGGAAAAATAAAAACAGACAAAAAAAAAGGAAAGATTGGTT
  CTCCCAGCACTGCTCAGCAGCCACAGCCTCCCTGTATGCCTGTGCTTGGTCTACTGATAAGCCCTCTAC
  AAAAAAACAAAAGTATATATATATATGTACATAATATCAGAATTATAACAGGCAAATAAAACCTGAAAA
  TCAAGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001143963.2
• Summary: The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]
• Locus ID: 3897
• MW: 41.9