CDC6 (NM_001254) Human 3' UTR Clone

CAT#: SC212842

3' UTR clone of cell division cycle 6 homolog (S. cerevisiae) (CDC6) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CDC6
• Synonyms: CDC18L; HsCDC6; HsCDC18; MGORS5
• ACCN: NM_001254
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC212842 3’UTR clone of NM_001254
  The sequence shown below is from the reference sequence of NM_001254. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGAAATATCTTAGCTACTGGATTGCCTTAAATTCTTCTCTTACACCCCACCCGAAAGTATTCAGCTGGC
  ATTTAGAGAGCTACAGTCTTCATTTTAGTGCTTTACACATTCGGGCCTGAAAACAAATATGACCTTTTT
  TACTTGAAGCCAATGAATTTTAATCTATAGATTCTTTAATATTAGCACAGAATAATATCTTTGGGTCTT
  ACTATTTTTACCCATAAAAGTGACCAGGTAGACCCTTTTTAATTACATTCACTACTTCTACCACTTGTG
  TATCTCTAGCCAATGTGCTTGCAAGTGTACAGATCTGTGTAGAGGAATGTGTGTATATTTACCTCTTCG
  TTTGCTCAAACATGAGTGGGTATTTTTTTGTTTGTTTTTTTTGTTGTTGTTGTTTTTGAGGCGCGTCTC
  ACCCTGTTGCCCAGGCTGGAGTGCAATGGCGCGTTCTCTGCTCACTACAGCACCCGCTTCCCAGGTTGA
  AGTGATTCTCTTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTGCCCACCACCGCGCCCAGCTAATT
  TTTTAATTTTTAGTAGAGACAGGGTTTTACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCCTCAAG
  TGATCTGCCCACCTTGGCCTCCCTAAGTGCTGGGATTATAGGCGTGAGCCACCATGCTCAGCCATTAAG
  GTATTTTGTTAAGAACTTTAAGTTTAGGGTAAGAAGAATGAAAATGATCCAGAAAAATGCAAGCAAGTC
  CACATGGAGATTTGGAGGACACTGGTTAAAGAATTTATTTCTTTGTATAGTATACTATGTTCATGGTGC
  AGATACTACAACATTGTGGCATTTTAGACTCGTTGAGTTTCTTGGGCACTCCCAAGGGCGTTGGGGTCA
  TAAGGAGACTATAACTCTACAGATTGTGAATATATTTATTTTCAAGTTGCATTCTTTGTCTTTTTAAGC
  AATCAGATTTCAAGAGAGCTCAAGCTTTCAGAAGTCAATGTGAAAATTCCTTCCTAGGCTGTCCCACAG
  TCTTTGCTGCCCTTAGATGAAGCCACTTGTTTCAAGATGACTACTTTGGGGTTGGGTTTTCATCTAAAC
  ACATTTTTCCAGTCTTATTAGATAAATTAGTCCATATGGTTGGTTAATCAAGAGCCTTCTGGGTTTGGT
  TTGGTGGCATTAAATGGCAATTTGTGACTGAGACACCAGAGGGCACCCTTATAACATTGACTACTTTGA
  AAGGGGATGCTTGAGGCTGAGGTAGTTTTGAGGGAAAGGGGGAAGTTCTAGTTTGATTTGTTTGGGAGA
  TTGCTGTCCTTTCATAACTGAAGCAAATGCTTGAAATGTAAACCATGTGTGATACGAATGATCTGAGAG
  CCAGTGACCAAGTCTGCTTTTCCCTGAGCTCTCCTGCTGCCGCCTCTACCTTTCTGACATGGCCTTCCA
  TTTGGAACTTGATATGTGTACAAGTGATCTGTTGGCCACTGTTTCATTCATCCTGACCACTGTATCCGT
  GTAATGTGATCTGAGCTACAACAGGTGGTATGGGATAGAGGCAAGGAAAGCAGAGAGAGCCCTCTGAAA
  TGAACACTACCCACTGGTGTGTGTAGCACATGATAGTCTGTAGGCATGAGTCTGAATGTTGTTCTCACA
  CTCCTTTACTAGAGTATTCGTTCCCTCTGAAGGTAGTTTGAACATGGAAACACAGTTCTATACCTAGAA
  GGTTGGAAAGTGTCTGGGACTATAAGGGTTAACATGCATAATTGCAAAGATTGTCCGAAGGCTGATGGA
  AAGCAGAGACTGAATGGGATTGAGAACAGATGCGAAGCTTGATTTAAATTACATTTTATTGGATGCTGC
  AGCCTTAAGAGACGTGACTGCTTTACAGTTTGTTTCCACACTGTGGGCAGCTGCTGTCTGTTCTGTGTC
  CACAGTAGGATCCTGCCCAATAAGGAGCAGCCTCCCCACCTCATTGTGTTTGAGGCTTGGCGCCTTCC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001254.4
• Summary: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]
• Locus ID: 990
• MW: 75.9