PCDHB7 (NM_018940) Human 3' UTR Clone

CAT#: SC212837

3' UTR clone of protocadherin beta 7 (PCDHB7) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PCDHB7
• Synonyms: PCDH-BETA7
• ACCN: NM_018940
• Insert Size: 1196 bp
• Sequence Data:
  Insert Sequence

  >SC212837 3’UTR clone of NM_018940
  The sequence shown below is from the reference sequence of NM_018940. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CGCCCATTTCAGAATAATTTGGGTTTCTGATAAAGAATGTAAACTAAATCCGCGTCTGTGAATACGTTT
  CTGATTAGGAACTTATTGCGAGGTTCCCTTAAGGGAGTGTCTTTACATCATTTCAAATATGTACTCTTG
  AAGTCAAGCAATAAATTTCTATACATAAAATAGGATCCTGATTTAGTATCAAGAACCCTTCACAAAGCA
  TGAAATGTATATGTGTAATGTTTTATGTCAAACAATTATGCTTAATATACAGTCTATTAAATGTAAGTC
  TTGTTTGAGATATTTTAAATTGCTTTCCATTGTTTTCAATCTCTACTGAGACTTCCTGAGTTGATTAGA
  AAGCTGTATGAGTGTACCTACCCTAGTCTCAGAAGCATAGACTGTAGAGTATCTTTTTAAGCATTTTTA
  AAAAATGCTTTTAATGCATCATACACTATTTTAACACTTTTAATCTGAGAAGAAGCATATGAGGCATGG
  TATTTTAGGAATGAACAAATAGATGGTCTTAGAGATTCAGTAAGTTCACTAAGTTCCACTAACTAATAA
  GTGACAAAACTGAGCATCCATCCCAGATCTGTCTGACTCTGGGTCAGTGACCCTGCTCCGATTCCATAC
  TGTTTTCTGTCATTAGATATCACCTGGCAAGTTTCTGCCTAATTAAGGAGAAGTCTTTTATCATATTTA
  TACTGCTGTCCAATCTTTTCTATATTTAGAAATAATAATGTACATATTTATCTATGGTTTTATTTTCTT
  ATACACCAAAAGTCCTGCTTTTCTGGGTCAATTTTCAACTATTATTACTAATGCTCTGATCTGTCCAAA
  CTCAAGCGGAAAACAAAATTGAAAGGGCAACCTGTGCCTTCTCCTTTCTTCAGAACATATGACTTTCAT
  TTCCCAGAAAAAAGATTAATGGTCCTGAGTAGGAATATTACATAATTTTGATTGCATCATTAGTTAATT
  ATTTTCTTCATATTGTAGATTTTCTGCAGTCACCCATACTTAACATTTGTAATACATTTTCCTGATTTG
  AAAGTTTGTTTTTAAAAGTTTTCTATTAATTATAGTGCACTATTGAATCAGGAAAATTTAAGAAAAAGA
  ATAGTTAGTTTTAAATGCATAATATCAAAGAGAATCATAGATGATCATTAAATTTTTAGAAATTCTCGG
  AAGTTAAGGAGAAGCACTGTTTT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_018940.4
• Summary: This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections. The transcript for this particular family member uses more than one polyadenylation site. [provided by RefSeq, Jul 2008]
• Locus ID: 56129
• MW: 47.2