PRPF4 (NM_004697) Human 3' UTR Clone

CAT#: SC212712

3' UTR clone of PRP4 pre-mRNA processing factor 4 homolog (yeast) (PRPF4) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PRPF4
• Synonyms: HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60
• ACCN: NM_004697
• Insert Size: 1275 bp
• Sequence Data:
  Insert Sequence

  >SC212712 3’UTR clone of NM_004697
  The sequence shown below is from the reference sequence of NM_004697. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGGACCTTCAAGCTGTGGATGGCTGAATAGATGACAATGGGAAAAGGACTTGAACCTCAAGCTCTCTCT
  AAGGAGCTGTTTTCCTCAAACGAGAAGAATTGAAGTGTTTAGTTCTATCATGTTTTCTGCCAATTACCA
  TGCATAGACCCTCAGTAGAATTGGATTTCCATGTCAGCCCCCACTCCAGGAAGGCAGCCCAATCCCTAG
  GTGATGGGGAACCCCTCTCACGGTTGAAAATTTATTACCTTTTTACGCCCTGCCACGAACTGTGTAGAC
  ATTGTTTTTATTAATCTTTTGTTTGGCCGGGCGTGGTGGCTCACGCCTGTAATCCTAGCACTTTGGGAG
  GCCGAGGTGGGTAGATCGCTTGAGCTCAGGAGTTCAAGACGAGCCTGGGCAACATGGCAAATGCCGTCT
  CTGCAAAAAAATACTAAAATTAGCTGGTCGCGGTGGCTTCTGCCTGTGATTCCGGCTACTTGGGAGGCT
  GAGGTGGGAGGGATTGCTTAAGCCTGGGAGGCAGAGGTTGCAGTGAGCCGAGATTGCGCCATTGCACTC
  TAGCCTGTGTGACAGAGCAAGACCCTGTCTCAAAAAAAAAAAAAATTTGTTCGAATGCCTTATAGCCTT
  CCTCACAGCACCCAGGATTGTGACTGACTCTGCATTTTTAATTCTTGAAACTTGGCTTTCCATAACATG
  GTACATGCTTCAGGACTACATATGACCCAGAGAGCAAGGTGGCTGAACTATAGTCTGGAAGCCCTCAGG
  TAAAGAGGCACATCTCACCACTCATTGGTTAAACAATGCATCATAGCGAGCACTTTTCCTTTCCCTGGA
  GAATGGGATGTGAAGCAGTAGACCGCAGCCACGCCGATGGTTATACAGTGAAGAAGACTTCACCTCTTC
  CTATTGAGTTTGCTTGGAATGCTGACAGCATCAGGCAACTCTGAACTGAACATTTGCTTTGTCAGAAAA
  TATCTTTTTTTTTACTTTGAAGTTTGGCAACCTTCATGTTACCCCAAAGCAAAACCATTGTGTCAGGAG
  TCAAACAAATGTTTAGAAAGCAAACATGACGTCTCTATTGTACAACCTCCTTTCTCTTGGCTGTTTAAA
  GGATGTACTTCGTGTATTAAAGGGTACTTTATGTTGAAGTAACGAAAAAACCCTAATGGGTGTTCCTAA
  ACCTAAACCTTTTCTTTATTCCACATTTGCTACGGTAAAATCCTGTTACAAAACTACCCTATAAAGAAT
  TATTTTCTATAGTTAAGCATTTTCTGAATCCTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004697.5
• Summary: The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]
• Locus ID: 9128
• MW: 48.8