ATP6J (ATP6V1G1) (NM_004888) Human 3' UTR Clone

CAT#: SC212651

3' UTR clone of ATPase H+ transporting lysosomal 13kDa V1 subunit G1 (ATP6V1G1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ATP6V1G1
• Synonyms: ATP6G; ATP6G1; ATP6GL; ATP6J; Vma10
• ACCN: NM_004888
• Insert Size: 1166 bp
• Sequence Data:
  Insert Sequence

  >SC212651 3’UTR clone of NM_004888
  The sequence shown below is from the reference sequence of NM_004888. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ATCCATGAAAACTACCGCATAAATGGATAGAAGAGAGAAGCACCTGTGCTGTGGAGTGGCATTTTAGAT
  GCCCTCACGAATATGAAGCTTAGCACAGCTCTAGTTACATTCTTATGATATGGCATTAAATTATTTCCA
  TATATTATATAATAGGTCCTTCCACTTTTTGGAGAGTAGCAAATCTAGCTTTTTTGTACAGACTTAGAA
  ATTATCTAAAGATTTCATCTTTTTACCTCATATTTCTTAGGAATTTAATGGTTATATGTTGTCTTTTTT
  TCCTATGTCTTTTGGCTCAAGCAACATGTATATCAGTGTTGACTTTTTCTTTCTTAGATCTAGTTTAAA
  AAAAAAAAAAACCACATAACAATTCTTTGAAGAAAGGAAGGGATTAAATAATTTTTTTCCCTAACACTT
  TCTTGAAGGTCAGGGGCTTTATCTATGAAAAAGTAGTAAATAGTTCTTTGTAACCTGTGTGAAGCAGCA
  GCCAGCCTTAAAGTAGTCCATTCTTGCTAATGGTTAGAACAGTGAATACTAGTGGAATTGTTTGGGCTG
  CTTTTAGTTTCTCTTAATCAAAATTACTAGATGATAGAATTCAAGAACTTGTTACATGTATTACTTGGT
  GTATCGATAATCATTTAAAAGTAAAGACTCTGTCATGCATTTTTCCCCATTCTTTTTTTTTTCCCTGTC
  TCCGTGACAACCAGTGGTTCTTCATTTTTGATCATGCGAAATGCATTTTGACCCAGATGGTCTGCAGAA
  CTTCACTTAGGACATTAGCACACAAATAGCACACATATCACATACCCATTTATATACATAATTAGAAAA
  TGTTCCTGATACATGTGTCATGTGATTTCTTCCAACAAATGTTTTGGAAAACAGCAAGCAACTAGTCTG
  TAGGTTGTCTTTTCTCTAGAGATCCTTGTCCTGGGACAGCCTGGGGCAAGTTATTAAGCATATTTCCTT
  CCTGGTGGGCTAGATGATGTGCATTATACCTGTAGTACCTTCATACTAGCCCTTACAAGTTAAATGTCC
  TGTGGCCATCTTAGCCGAAAGTCTTTGGTTGCAAAATCTTATAGGTAAAAACAGTTGAAAGAACATTAT
  GGGATTTCCTTGCTTGTTCTGATACAATACAACTAGATGACCTTAAAAACCCTCATATTTAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004888.4
• Summary: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of three V1 domain G subunit proteins. Pseudogenes of this gene have been characterized. [provided by RefSeq, Jul 2008]
• Locus ID: 9550
• MW: 44.7