TFIIA2 (GTF2A2) (NM_004492) Human 3' UTR Clone

CAT#: SC212216

3' UTR clone of general transcription factor IIA2 12kDa (GTF2A2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TFIIA2
• Synonyms: HsT18745; T18745; TF2A2; TFIIA; TFIIA-12; TFIIA-gamma; TFIIAS
• ACCN: NM_004492
• Insert Size: 1100 bp
• Sequence Data:
  Insert Sequence

  >SC212216 3’UTR clone of NM_004492
  The sequence shown below is from the reference sequence of NM_004492. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAAAATACTGGCTCCAATACTACAGAATGAATAGAAAAAATATGACTTTTTTACACCATCTTCTGTTAT
  TCATTGCTTTTGAAGAGAAGCATAGAAGAGACTTTTTATTTATTCTAGAATTGCAGAAATGACTACACT
  GTGCTATACCAGAGAATTCCAGTAGAAAGAAACTTGTAACTCTGTAGCCTCTTACATCACCTTTATTAT
  ACAGCATGAAAAACCATAACTTTTTTTTAAGGACAAAAGTTGTTGCCTTCCTAAGAACCTTCTTTAATA
  AACTCATTTTAAAACTCTGAGTAATAACTGGTGAAGCTATTAAGGTAGCAATATGAAGATATTAGATAA
  TGCATTTTCCCATTTCCTTTGCAAGGAACATGATGCCCTTTCAGAATGCTTATTTCAATAAAGTCATGT
  TTTGTCAGTTGTAACTTTACCTGATGAAAGCATCAGCAGGGGCAAAACCAAACTCTGGATGGAATTTTT
  TAAGAAAATCCCTTGCTTTTTAAAATACAAAACCAAAGTTAATCATTGGTATAAAAAAACCCAAGTACA
  ACTTGATTAGGAAAAGTTAGACAATCATAGCAAAAACAAAACCAAAAAACTTTCCTTCTTAGACCAGGT
  AACATGAGGAAGGGGGAAAAAAACCCAAACAAAATAGTCCCCCCACATGACAGGTGTCTTCAGCTTGCC
  ATATTGATCCAAAGTTCATACATTTTATCATGTACAAGATACATTAGACTTACAATAAAGTCTAATTAT
  TAGACTTTTATGTCAGTGGATTGTGCTTGCAGAAATACAGAAGTAACATACTGACCCTAGGTGAGGAGT
  TTCTTACATGGTTAACTGGATTGACCCAATTGGAGTAAAAGATTTTTGCTGTTACCTCGATGCTCCTGC
  AGTTGTTACCTCAGCATGCCACTGTAGTCCTTGACTCTCACATTAAAACTAACTTTGGCTAGGTACTGG
  TGGCTCACACCTGTAAGTAATCCCAGTACTTTAGGAGGCTGAGACTGGAGGATTACTTGAGTCCAGGAG
  TTGGAGACCAGCCTGGCCAATATAGACTGTCCCTACAAAGAAAATAAAATTGTTTCATCTTCCAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_004492.3
• Summary: Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).[supplied by OMIM, Mar 2008]
• Locus ID: 2958
• MW: 42.5