TLR5 (NM_003268) Human 3' UTR Clone

CAT#: SC212140

3' UTR clone of toll-like receptor 5 (TLR5) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TLR5
• Synonyms: MELIOS; SLE1; SLEB1; TIL3
• ACCN: NM_003268
• Insert Size: 1081 bp
• Sequence Data:
  Insert Sequence

  >SC212140 3’UTR clone of NM_003268
  The sequence shown below is from the reference sequence of NM_003268. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCGTTGCAAACTGTAGCAACCATCTCCTAATCAAAGGAGCAATTTCCAACTTATCTCAAGCCACAAATA
  ACTCTTCACTTTGTATTTGCACCAAGTTATCATTTTGGGGTCCTCTCTGGAGGTTTTTTTTTTCTTTTT
  GCTACTATGAAAACAACATAAATCTCTCAATTTTCGTATCAACACCATGTTCTGTCTCACTAACCTCCA
  AATGGAAAATAATAGATCTAGAAAATTGCAACTGCCCTTAGAGGTTTCCAGTCTCCATTGATTTTCTTT
  CAGATCCAATAATACCGTTCTGTCCTGCTGTGTTGATTATGGAATGTATCCTAATCATGGGAAGGGCAC
  CTTGGGAGAAGTTGCAGATGGCTGACGTGCTTTCTCTAGCATTCAGCTAAAAAATGGGATGGTCCATGA
  TTCTGCGTTCTCTGTGTTCTGCAAAACAACATTAAGTAGAAAACAAACAGAAGCAGAGGCACATTTCCT
  TCTTTTGCCACAGAAACAATGCCACTGTTGAGTGCAAGTCACACTTTGTCTCATAGATAAGAGGTGGCC
  CCAAAGAAGCTGGGATGAATGAGTCACTACCTGTCTTGTGACTGTACTGCACTTCATGTTCTTACCTTC
  GGCTTCTCCAGGTCTCGCCCTAGTGAGCCAAGAACTTTCTCTCACATGCTGCCTTTATTTAAGCCTGGC
  CTTCAAGACCTTCCATGATTTATCACCAACCTACCTTTTCAGCTTTGTTTCCTAGCACACCTCATTGAT
  CAGCTGCTCAGGCTGTTGCATGAAGAACATGGACTTTGCACACAGATCCTGGGTTGAGTTCTGAGTCAG
  CTGTGTATTAGCCATGTAACCTTGGCCTTGGTATGCCCTTGCTGGACCTACATTTTCTCACGTAACAGC
  ATCTATGTCATAGAATTCCTGTGAAAATTAAATTGGCCAAGGATGTCAGGGCTTCTCAGATCTTTTCCT
  TCGTTGCCCTAATGACCACAAGAGAACACATACACTGAAGGCCTCCTGGGGGGCAGTTGCAATTTCACT
  GAAGTTGTATTTTTTTATCTTAAATGAAATCTATGTGTATTTTCCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003268.6
• Summary: This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009]
• Locus ID: 7100
• MW: 41.1