DMP1 (NM_001079911) Human 3' UTR Clone

CAT#: SC212052

3' UTR clone of dentin matrix acidic phosphoprotein 1 (DMP1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DMP1
• Synonyms: ARHP; ARHR; DMP-1
• ACCN: NM_001079911
• Insert Size: 1067 bp
• Sequence Data:
  Insert Sequence

  >SC212052 3’UTR clone of NM_001079911
  The sequence shown below is from the reference sequence of NM_001079911. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GATGACAATGACTGCCAAGACGGCTATTAGCATCAGCTGTCCTAAGAAGCAGTTGTCACATAAAGGAGT
  CTTAGGGACTTGAAAATGTATCATGATAACTATAATTTATTGATGTTTTGATCAAAAGAATAACCAGAT
  GCCATATTTTTCCTGAAAGGAATTGCTGGACATTACACTTGTTTTTAGGGTGTCATCATTTCACAGAGG
  TTTAAATACTGTGGAGTGACACCAGAACACAGCCAAAGAGGCTAGAAGCAAGAAAGGATCTGCATGATA
  ACTTTGCAGCTGAGATAGTTCCTAATTCATCAACGTAACAAACAAAGCTATTGGGTGTCCATGATATAC
  CAGGCACTATGCTAGGTGTTGAGAATGTAAAGCAGGTTAAGACTTGTTTCTTGCTCCTGAGGAGCGTAT
  AGAAGGACCCACAAAGCTACAGAGTTAAAGAGGGATATGTATAAGAGAAACAAGAATTGTTACAACCCA
  GTATGGTGAGTGCCAAGACAGAGAGCTATGAACACGATATCTATCTGGGAACACTGAGAAGGGTGACCA
  ATGCGTTGGAGAACGAGGGAGGGCTTCATAGCAGAAGACATTGGAGTTGGAAGGTTGCATAGGGTTTCC
  TCATTGGAGACCGGGAAATTCTATTCTTGGGATAAATAATAGTAAATTCTATAGGATTCAATACTTAGT
  TTGCAAAAGCACTTTGAAATTATGGCACAGTGAGTTGTTTTGGATAAGAAAATCTTTTTCTCCCAATTA
  ATTTACCAATTCATCATTTTTTTTTTTTTGTAGAACTCCCATAAACATCACCTTACTAATCACTGGTGA
  TGATAAGAAGGATTGGGTCAGGAAGAGTGGGAGAAAGAAATTCCTCTTTACGTAGATACTTTTTAGCTT
  TATTTTTTCTAAAATCAGTTTGCGTGCAATGCTAGAAAAAAACTGTTCTCTGAGTCCTTTACAGAGCAA
  AATTCTGTATGTAAGATTCAATTGATTTTTGACAAATACCATTTGAAATATTACCTCAACATAAAATAC
  TTGTTTTGTAATAAAGATTATAATACCCAGAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001079911.3
• Summary: Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 1758
• MW: 42.6