HERC4 (NM_015601) Human 3' UTR Clone
CAT#: SC212050
3' UTR clone of hect domain and RLD 4 (HERC4) transcript variant 2 for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | HERC4 |
ACCN | NM_015601 |
Insert Size | 1056 bp |
Sequence Data |
>SC212050 3’UTR clone of NM_015601
The sequence shown below is from the reference sequence of NM_015601. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC GATCACAATGAAGGCTTCAGTTTAATATAACTTTGGAGTTATAACTATTCAGTTTAGTGCAAAAGCATT AAACTATTTGTGTTTTTCTTGTGGTGATGAATTCAGCAAGGTGACAGAGGTACTATTATAATTCTTACT TGCAGAATGTTCAATCTACGAGTGTTCATGGAAGCCAAAAAATATTAAAGGAAAATGAACAAACTGTTA ATATTATTGTACAGAACCATGGATTTTTTTTGACCATCTTCTAATAAACATAGCAAGTATTATGAATAC ATTAAAGTTTTACTAACATGAATTTTAAGAGTTTGCATATTTCAGAAATGATCTGGTGTGAGTGCATGG AAATATTGCTTAATTTTTCTTCAATCATTGAGTGAAAAACCTTTAACTTTGGCCTGCAATAGTCATTTG ATTATTTTTTCATTTTGTAAATAATGTTAAGTTTTGTAATAAAATAGTTATGTTCTGATACCAGTACAG TTTCTATGGTTGTAATTGAACTTGAACAGACTTTTAAAGGTTAAAAATTATGATTTAAATCTTACTCTG AGACTCTATAAAAAGAAAAAAAAGGTAGCATGGTGGAAACACTATCTTTTCTTTTTTGCTAGAATAAGT GTCTTTGTGCAAACCTAAATCACAGATAGGGAATATACTACATAACCTGCAGTTCTTTTCTTTGTGAAT CCTGACACACTGATAGATGGGGGATTGTCGATCAGAGAACTTATTAATATTTAGTACTGGAAGAACTCT GTCTCCACAGTTGCCAGTAATAAAAAGAAACATTGGCTACTATGAGCACCAATCACTGGGTTATAGCTT TCAAAATTATTGATGCTGCAGTGCTTTAGAGCTATTTCCTTGAATTTAAGAAACAAATCTTAACAGTTT TATGGTGCTGATGCTTAGTTGTCTCATGCCATTAAATTGTAAAAGTGAGTTGATGCAATACATGTGACT TTCTGCTATAATGCAAATATTTATTTTTTAAATTTATTTTAAAATGCCGTGAAAACTGTTTAATAAAGA TTTATTGTTTTAATATTTAAA ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_015601.4 |
Summary | HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008] |
Locus ID | 26091 |
MW | 41.5 |
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