CNOT1 (NM_016284) Human 3' UTR Clone

CAT#: SC211909

3' UTR clone of CCR4-NOT transcription complex subunit 1 (CNOT1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CNOT1
• Synonyms: AD-005; CDC39; HPE12; NOT1; NOT1H
• ACCN: NM_016284
• Insert Size: 1037 bp
• Sequence Data:
  Insert Sequence

  >SC211909 3’UTR clone of NM_016284
  The sequence shown below is from the reference sequence of NM_016284. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CAAGTAATGGAAGGGACAGGTGCCAGTTAGACGAAACTGCATCTCTGTTGTACGTGTCAGTCTAGAGGT
  CTCACTGCACCGAGTTCATAAACTGACTGAAGAATCCTTTCAGCTCTTCCTGACTTTCCCAGCCCTTTG
  GTTTGTGGGTATCTGCCCCAACTACTGTTGGGATCAGCCTCCTGTCTTATGTGGGCACGTTCCAAAGTT
  TAAATGCATTTTTTTGACTCTTGGCCAAAATTTAGAAGATGCTGTGAATATCATTTTGAACTTGTGTAA
  ATACATGAAAGAGAAAACCTTTGTCTGGAACTTCTTGGCTTTGTGCAAGCTGTGTCCAAGGCAAGTACA
  TAAACTGGTACCTTGTAATGAAGAGGCAGCTGATGCCATGCACTTGTCTGAGGGCATAGCTCCATGTCT
  TCTGACATTCCTGGTGTCCCAAAGAATAGCAAAAAGCCAGTTTGAATATTATGTAACTTATTTTTTTAA
  TGTGGACAGGGGACCTTGAAAATCACTAAGTTATTAAAAATGTGGATGTGCTAGAATTGGATATGTCCA
  GGAACATGGGAAGGGCTCACTATTGGAATCCCATGAGTTTCCATTTTGTCTCTACCCAAACGTATTCCA
  AAGCTGACTGCATTTGTACCATCTTATTTCTTTTGGGGATTATACACCTCAGCCGCCTGAGATGGGGGT
  CAGCTCTTTATATAAAGGGAAACCAGACCAGGCCTAAAGCCCACCCCTACCCTCACCCCCCCAATCCTC
  TCCTGAAACTTAAAAACAGTGGGAATATAGGAAAGGGAACCAAATCTCATTAATTAATTGTTCTCCCCC
  ATTACCCCACTGAATGAATGGCCATACAGGCTAAGCTGAATAATGACAAAGTTGAAAGGACCAATACAG
  CCCCTTTTATAAGGATTTTGAATGTTTTGCAAATGTATTGGTCCCTGTGTTGTATTTTGTAGCCTTTTC
  CTGGGCTTCAGCTCCCCTACTTCTTGTATGTGTATGCATACTGTAGCTAACCATTAAAGTCATGACACA
  CA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_016284.5
• Summary: Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3' UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity.[UniProtKB/Swiss-Prot Function]
• Locus ID: 23019
• MW: 38.3