DUSP10 (NM_144729) Human 3' UTR Clone

CAT#: SC211598

3' UTR clone of dual specificity phosphatase 10 (DUSP10) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DUSP10
• Synonyms: MKP-5; MKP5
• ACCN: NM_144729
• Insert Size: 1022 bp
• Sequence Data:
  Insert Sequence

  >SC211598 3’UTR clone of NM_144729
  The sequence shown below is from the reference sequence of NM_144729. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAGCTGATGGGCGTGGAGACGGTTGTGTGACAATGGTCTGGATGGAAAGGATTGCTGCTCTCCATTAGG
  AGACAATGAGGAAGGAGGATGGATTCTGGTTTTTTTTCTTTCTTTTTTTTTTTGTAGTTGGGAGTAAGT
  TTGTGAATGGAAACAAACTTGTTTAAACACTTTATTTTTAACAAGTGTAAGAAGACTATAACTTTTGAT
  GCCATTGAGATTCACCTCCCACAAACTGACAAATTAAGGAGGTTAAAGAAGTAATTTTTTTAAGCCAAC
  AATAAAAATATAATACAACTTGTTTCTCCCCCTTTTCCTTTTAAGCTATTTGTAGAGTTTATGACTAAA
  TAGTCTGTGCAGGTTCATAGACCGAAGATACTACACACTTTAAACCAATTAAAAAGAACCAAAAGTAAA
  TAGAAAAGACATTGAATCACCAAGGCCTGGGATCAACCTGGGCTGTCCACACAGAAAACAAAAACCCAA
  CCAAACCAAGCCCTGTTGTGCTCACTGGTGCAAAGAGAAGATCAGGGCAGCTTAAGTGGTCTAAGAATC
  CTTCAGGCATTCTTTAAGGAGAAAAAGGATACCTTTGATTTTGTGTGTTTCATGCTCTGGATTTTTTTT
  TTTTTTCCTTCTCTGGGTTTAAGAGATTTTTTTTGAAATAGTGAGGAACTGACCATTATATGCCTTCAC
  TGGCTTCTTGTGCAATAATATGATGTTTTAAGTGTGCAAACAAGTTAGAGCTGGCAGCTGAATGATAGA
  CAAATAGTGCAAATTTGCCAGCTTGGAGATAGAAAGGAATTCAACAATATATCAAATACTTTCCTTCCC
  ACCTTTTTCCTTTTTTTTTTTTTTTTCTGATTTGATTCTGGTTACAGTGCCATAAACCTTGTTACATAT
  GTATATCAGAATGTAAGAAAAAAAAATTTATTTAAAAATATTTTTCGCAAAAAAAAAAAAAATCTTGGT
  GTGTTTCTGTTGATTGTGTAAAAATTTATTTTCATTATATAAATGAAACTGGTTAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_144729.2
• Summary: Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
• Locus ID: 11221
• MW: 40.4