ZCRB1 (NM_033114) Human 3' UTR Clone

CAT#: SC211539

3' UTR clone of zinc finger CCHC-type and RNA binding motif 1 (ZCRB1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ZCRB1
• Synonyms: MADP-1; MADP1; RBM36; SNRNP31; ZCCHC19
• ACCN: NM_033114
• Insert Size: 1011 bp
• Sequence Data:
  Insert Sequence

  >SC211539 3’UTR clone of NM_033114
  The sequence shown below is from the reference sequence of NM_033114. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TTCAGTGATGAGGAAGAACTTAGTGATTAAAATCTTGCCCCAGCACAGTAATAAAAATCAAGATTTGTT
  AGTAACAATCTTGAAGAGCTAATTTTAATAAAAATAAGAAAAATTAATACTATCATGTTAATACTATTA
  TTGTCATCCCAAGAAAAAAGATATTTTAAAAATTTATTTGAAAAGTTCATTATAAGGGCTTTATTCATG
  CCTGATTTGTTTACATGAGGACTTCTGAAATTAATCCTTAAAACAAACTTCCTGAAGACCGAAAAGTTG
  AATGATTTATTGTTACTTATATTAATAAACTTTTCAAGAGAATTTTGTCTTTAAATATGGGTGTTTTGT
  CATCATATTTCTTGTAGCTTTATCCCAATCTGGATAAATTGTAAATACCTATAAAATAAATTATAAATA
  CCTATAAAATATAAAGTAACATAGCTCTAAAAGGCTTAAAATCAAACACAGGTGTTATTTGTCTGCCCT
  ACCCATAGCACCAAATTCCATTCCCTAGAAGAAACTACTTACATGTGCACACATGTAGCTAAATAAAGT
  ACATATATTTCAGTCACTTAATAAAAGAATAAAGGAGAATTATTCAATCTCTTATACTTCTCCTACCTT
  CCTCAATATTCCCAATGTGGCTGTATTAAAAATTTGGGGGAATCCATATATATCTTTTTTAATAACCAA
  GTAAATACTTTTCATTTCTGAGCCAAGGAATATGCTATGATTACGTTTTTTCCTAGAGTTAATAATTGT
  CTATTTTTTTTCCATGTATTGTCTTTGTATTTATGACTAAATCTTCCCATTCTGTCTGCAGGTGGGTAT
  ATGGTAATGGGATTAGAGAGCCTTTAATTTTCTGCTTTGTATATTTCTATATTGTTTAACTTTGTAAGA
  ATGCCCATTACTTTTTTAACTAGTAAAAGCAATAGAAATAAGTTAATACTATCATAGTAATATTATTAT
  TGTCATCCCAAGAAAAAATATATTAAAAAGATATTTGAAATTTCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_033114.4
• Summary: Pre-mRNA splicing is catalyzed by the spliceosome. U12-type spliceosome binds U12-type pre-mRNAs and recognizes the 5' splice site and branch-point sequence. U11 and U12 snRNPs are components of U12-type spliceosome and function as a molecular bridge connecting both ends of the intron. The protein encoded by this gene contains a RNA recognition motif. It was identified as one of the protein components of U11/U12 snRNPs. This protein and many other U11/U12 snRNP proteins are highly conserved in organisms known to contain U12-type introns. These proteins have been shown to be essential for cell viability, suggesting the key roles in U12-type splicing. [provided by RefSeq, Jul 2008]
• Locus ID: 85437
• MW: 39.7