RAD51 (NM_001164269) Human 3' UTR Clone

CAT#: SC211534

3' UTR clone of RAD51 homolog (RecA homolog E. coli) (S. cerevisiae) (RAD51) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RAD51
• Synonyms: BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51A; RECA
• ACCN: NM_001164269
• Insert Size: 1192 bp
• Sequence Data:
  Insert Sequence

  >SC211534 3’UTR clone of NM_001164269
  The sequence shown below is from the reference sequence of NM_001164269. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GCAGATGGAGTGGGAGATGCCAAAGACTGAATCATTGGGTTTTTCCTCTGTTAAAAACCTTAAGTGCTG
  CAGCCTAATGAGAGTGCACTGCTCCCTGGGGTTCTCTACAGGCCTCTTCCTGTTGTGACTGCCAGGATA
  AAGCTTCCGGGAAAACAGCTATTATATCAGCTTTTCTGATGGTATAAACAGGAGACAGGTCAGTAGTCA
  CAAACTGATCTAAAATGTTTATTCCTTCTGTAGTGTATTAATCTCTGTGTGTTTTCTTTGGTTTTGGAG
  GAGGGGTATGAAGTATCTTTGACATGGTGCCTTAGGAATGACTTGGGTTTAACAAGCTGTCTACTGGAC
  AATCTTATGTTTCCAAGAGAACTAAAGCTGGAGAGACCTGACCCTTCTCTCACTTCTAAATTAATGGTA
  AAATAAAATGCCTCAGCTATGTAGCAAAGGGAATGGGTCTGCACAGATTCTTTTTTTCTGTCAGTAAAA
  CTCTCAAGCAGGTTTTTAAGTTGTCTGTCTGAATGATCTTGTGTAAGGTTTTGGTTATGGAGTCTTGTG
  CCAAACCTACTAGGCCATTAGCCCTTCACCATCTACCTGCTTGGTCTTTCATTGCTAAGACTAACTCAA
  GATAATCCTAGAGTCTTAAAGCATTTCAGGCCAGTGTGGTGTCTTGCGCCTGTACTCCCAGCACTTTGG
  GAGGCCGAGGCAGGTGGATCGCTTGAGCCCAGGAGTTTTAAGTCCAGCTTGGCCAAGGTGGTGAAATCC
  CATCTCTACAAAAAATGCAGAACTTAATCTGGACACACTGTTACACGTGCCTGTAGTCCCAGCTACTCG
  ATAGCCTGAGGTGGGAGAATCACTTAAGCCTGGAAGGTGGAAGTTGCAGTGAGTCGAGATTGCACTGCT
  GCATTCCAGCCAGGGTGACAGAGTGAGACCATGTTTCAAACAAGAAACATTTCAGAGGGTAAGTAAACA
  GATTTGATTGTGAGGCTTCTAATAAAGTAGTTATTAGTAGTGAATGTGCTGTTTATAGCAATTATTGCA
  GTGCAAGCTATTTCAAGACAGGGTTTCCATAATCTTTTTGGCACTGTATAGGGGTGATCAGTTTCTGTT
  GCTTCAAGATTTGAAACCAGAAAGGAAAGTCCCACTTGCAGATGATTGTGCTTAAAAGCTAATGGAAAA
  ATAAAAGAAAGACATACCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001164269.2
• Summary: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
• Locus ID: 5888
• MW: 44.6