USP27X (NM_001145073) Human 3' UTR Clone

CAT#: SC211341

3' UTR clone of ubiquitin specific peptidase 27 X-linked (USP27X) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: USP27X
• Synonyms: MRX105; USP22L; USP27
• ACCN: NM_001145073
• Insert Size: 964 bp
• Sequence Data:
  Insert Sequence

  >SC211341 3’UTR clone of NM_001145073
  The sequence shown below is from the reference sequence of NM_001145073. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GTGAAAGAAATGAACACACAAGCCTACTGAAGTGACACACAGACCTACCTGCAATGGAAGATGACGACA
  CCAATACTACAACTGGCATCGAGATCTAAAGGACCTACTTGACCATGGCCCATGGGCCTGACAGAGTAA
  GAATTGAACAGTACCCAGTGTCCAAAAGGTCCTGATTGGAAGAGAAATAGAAGGGGAGGGAGAAGAGGC
  TCTAGTCTAAGCAGTTGATGAAAGGGAAATTAAATGGTAGAAACACTCTGGGTGAGGAAGGCAGGAGCA
  GGAAAATGCTGACACTGGTACATATCCTATATTCCTCCAAAAGATTGTGTGGGAAAGTGTGGGGGGGAG
  GGGGGTGGTGGGGGGGGAGTGTGCCCTTGTAACCGTAAAACATCTTTCTCCATGGGTGTTTCAGCTTCA
  ATTGACCAATCACATAACAGTTATATGTTTGGGGGGAAAAAGAAAAGTTTCTAAAAAATGTAGCCCATT
  ACATATATGGGTATGAAAGCAGAAAAGTGGAGGAGGCAGGGATATCATTGACAAACACCTTTGCCAGTT
  TCTTTGTATTCGTGATTTTTTGTGCTATTAAATGCAGTATTAAAACAAGAAAAGCCCACCTGGGCTAGT
  GAAAGGAGAGGAAATAAGGGGGCTTGTAAAAGGGGAGCCGTCGCTCCAAAAGTAGAGAAGACAAGAAAT
  GCTGTAAGATAAGCAACCAACGCAGACAGGCCTCAGCTAAGAGTATAGTGTGTGTGTGTTAAAAAAAAA
  AAAAAAAAAAGAAAAAAAAAAGCTGTATGATATACTTGAGCAAATCAATGAACCTCTTTGCGATTGTTT
  TATCATCTGTAAAATGCAGATAATTCCTACCTTAAAGGGTTATTGTTAAAGATAAAATGATATAAAAAT
  GTGTCAAAGTATAAAGTAAAAGGCTTGGAACTTTGTAGGGACTCAGTAAACGTTTGTTGGATCCAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001145073.3
• Summary: This gene encodes a member of the peptidase protein family. The encoded protein functions as a deubiquitinase that is involved in upregulation of the pro-apoptotic Bim protein. This protein may act as a tumor suppressor by increasing levels of Bim to counteract anti-apoptotic signals in cancer cells. Mutations in this gene have been associated with X-linked cognitive disability. [provided by RefSeq, Dec 2016]
• Locus ID: 389856
• MW: 36.2