POLE (NM_006231) Human 3' UTR Clone

CAT#: SC211334

3' UTR clone of polymerase (DNA directed) epsilon (POLE) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: POLE
• Synonyms: CRCS12; FILS; IMAGEI; POLE1
• ACCN: NM_006231
• Insert Size: 965 bp
• Sequence Data:
  Insert Sequence

  >SC211334 3’UTR clone of NM_006231
  The sequence shown below is from the reference sequence of NM_006231. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTGCAGAAGAACCCACAGCTGGGCCATTAGCCAGCCCCGGGCCCCGGGTGCCTCTGCGTCCGTGCCAGG
  CCTCCTGATGCCAAGGCCACATCCCCGTGCTTCCAGTGACCAGACCACTGACCACCCTGACTGTCCAAA
  CCTGTGACCCCAGGCCAGGGAACGGGGAGGAAACCAAAGAAAACCATTTTCAGGGAGCTCAGACGTCAC
  AGGAGGGAGCGGGAGCAGGATGTGGCCCTGGCCTCGCCAGAGCACCTGAAGAAGCAGGCCGTGAGCGAG
  GCTGCGAGTGCCCTGGGCGCCGTTTCTCACGCAGTGAATGCTTTTCCAGGCCTCTGTTGCTTCCTGCAC
  CACACCTGGTGGGGTGGGAGCGTCCTCTAGGTGCCCCTAGTTCTTTGTCCTGCCTCCCAGAGGGAGGAA
  AAGCCCCTGGGGGCTTCTGGCTCCCTGAGATTGGGCTCTGAGACGAGACGGGTTCCCAAGGCCCTGGTG
  GGGCTGGAGTCTCACCTGTTTGCATGGAGAAATGGGCTGGCCCCACAGCCTCACAGGAGCAGTTTGTGG
  GCTGGTTTCCCCAGGAATCCAGACCCTAACCCGTGAGAATCTGGATTTTGGCTTGTGAGCCCTGCTTAT
  TTGGAGCCGGGTCTAGAGGGAACCCTCTATCAGCCTCAGGAAAACAAGACCTCTGTGCACCTCACTTTT
  GGCTCACTGCAGCCCTTGTCCTTCACCTCCACACAGGACCAGCTGGAAGCAGAAAGAAGAAAGGCCAAT
  TTCACAGGGCACCAAACAAGTATGAAATGTAAATCAGAAATGCAGACACCCCAGACGAGAGCCTCACAG
  GAGGGAGGGGGCCCCACAGGCTCCCCAGGAGGCTCGTGTCTTTGGCCCAGAGCCAGCCTTAGTTTGTCC
  CTGCCATCTACTGTCTGAGGCCATCGCTGCTACACTTTGTTTTTATTTGTATTTCATACTGAAGTTTC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006231.4
• Summary: This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013]
• Locus ID: 5426
• MW: 35