RPE65 (NM_000329) Human 3' UTR Clone

CAT#: SC211325

3' UTR clone of retinal pigment epithelium-specific protein 65kDa (RPE65) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RPE65
• Synonyms: BCO3; LCA2; mRPE65; p63; rd12; RP20; sRPE65
• ACCN: NM_000329
• Insert Size: 984 bp
• Sequence Data:
  Insert Sequence

  >SC211325 3’UTR clone of NM_000329
  The sequence shown below is from the reference sequence of NM_000329. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ACCTTTCATGGACTGTTCAAAAAATCTTGAGCATACTCCAGCAAGATATGTTTTTGGTAGCAAAACTGA
  GAAAATCAGCTTCAGGTCTGCAATCAAATTCTGTTCAATTTTAGCCTGCTATATGTCATGGTTTTAACT
  TGCAGATGCGCACAATTTTGCAATGTTTTACAGAAAGCACTGAGTTGAGCAAGCAATTCCTTTATTTAA
  AAAAAAAAGTACGTATTTAGATAATCATACTTCCTCTGTGAGACAGGCCATAACTGAAAAACTCTTAAA
  TATTTAGCAATCAAATAGGAAATGAATGTGGACTTACTAAATGGCTTTTAATTCCTATTATAAGAGCAT
  ATTTTAGGTACCTATCTGCTCCAATTATATTTTTAACATTTAAAAACCAAAGTCCTCTACACTTGATTT
  ATATTATATGTGGCTTTGCTGAGTCAAGGAAGTATCATGCAATAAGGCTTAATTACTAAATGTCAAACC
  AAACTTTTTCTCAAACCAGGGACTATCATCTAAGATTAATTACAGTAATTATTTTGCGTATACGTAACT
  GCTCAAAGATTATGAATCTTATGAATGTTAACCTTTCCGTTTATTACAAGCAAGTACTATTATTTCTGA
  TTTTATAATAAGAAAATCTGTGTTTAATCAACTGAGGCCTCTCAACCAAATAACATCTCAGAGATTAAG
  TTATATATTAAAAGCTTATGTAACATAAAAGCAAGTACATATAGTAGTGACTATATTTAAAAAAACAGC
  ATAAAATGCTTAAAAATGTAATATTTACTAAAATCAGATTATGGGATAATGTTGCAGGATTATACTTTA
  TTGCATCTTTTTTGTTTAATTGTATTTAAGCATTGTGCAATCACTTGGGAAAAATATTAAATTATTAAC
  ATTGAGGTATTAATACATTTTAAGCCTTTTGTTTTTAAATTTCTTTTCTTCCAGAGATTGTTTAAAAAT
  AAATATTGACAAAAATAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000329.3
• Summary: The protein encoded by this gene is a component of the vitamin A visual cycle of the retina which supplies the 11-cis retinal chromophore of the photoreceptors opsin visual pigments. It is a member of the carotenoid cleavage oxygenase superfamily. All members of this superfamily are non-heme iron oxygenases with a seven-bladed propeller fold and oxidatively cleave carotenoid carbon:carbon double bonds. However, the protein encoded by this gene has acquired a divergent function that involves the concerted O-alkyl ester cleavage of its all-trans retinyl ester substrate and all-trans to 11-cis double bond isomerization of the retinyl moiety. As such, it performs the essential enzymatic isomerization step in the synthesis of 11-cis retinal. Mutations in this gene are associated with early-onset severe blinding disorders such as Leber congenital. [provided by RefSeq, Oct 2017]
• Locus ID: 6121
• MW: 38.7